TABLE 1.
Strains and plasmids
| Strain or plasmid | Relevant properties | Reference |
|---|---|---|
| Plasmids | ||
| pMLH3 | Ampr Nbr Mevr; Haloferax volcanii-E. coli replicative vector | 15 |
| pGB70 | Ampr; pUC19 containing Pfdx-pyrE2 | 6 |
| pTA106 | Ampr; pUC19 containing Pfdx-trpA | Thorsten Allers |
| pTA131 | Ampr; pBluescript II with BamHI-XbaI fragment from pGB70 containing Pfdx-pyrE2 | 2 |
| pKD1 | pTA131 containing chromosomal tatAo region with Pfdx-trpA from pTA106 replacing tatAo gene | This study |
| pKD2 | pTA131 containing chromosomal tatAt region with Pfdx-trpA from pTA106 replacing tatAt gene | This study |
| pKD3 | pTA131 containing chromosomal tatCo region with Pfdx-trpA from pTA106 replacing tatCo gene | This study |
| pKD4 | pTA131 containing chromosomal tatCt region with Pfdx-trpA from pTA106 replacing tatCt gene | This study |
| pKD5 | Ampr Nbr; pMLH3 containing Pfdx-tatAo6HIS inserted in place of Mevr cassette | This study |
| pKD6 | Ampr Nbr; pMLH3 containing Pfdx-tatAt6HIS inserted in place of Mevr cassette | This study |
| pKD7 | Ampr Nbr; pMLH3 containing Pfdx-tatCo6HIS inserted in place of Mevr cassette | This study |
| pKD8 | Ampr Nbr; pMLH3 containing Pfdx-tatCt6HIS inserted in place of Mevr cassette | This study |
| pKD10 | Ampr Nbr; pMLH3 containing Pfdx-halocyaninIMYC inserted in place of Mevr cassette | This study |
| pKD11 | Ampr Nbr; pMLH3 containing Pfdx-halocyaninIMYC with the core twin-arginine codons replaced with lysine codons inserted in place of Mevr cassette | This study |
| pKD15 | Ampr Nbr; pMLH3 containing Pfdx-tatCtNterm-MYC inserted in place of Mevr cassette | This study |
| Strains | ||
| DH5α | E. coli F− φ80dlacZΔM15 (lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK+) phoA supE44 thi-1 gyrA96 relA1 | Invitrogen |
| DL739 | E. coli MC4100 recA+dam-13::Tn9 | 8 |
| H99 | Haloferax volcanii ΔpyrE2 ΔtrpA ΔhdrB | 2 |
| KD1 | H99 containing pKD5 | This study |
| KD2 | H99 containing pKD6 | This study |
| KD3 | H99 containing pKD7 | This study |
| KD4 | H99 containing pKD8 | This study |
| KD5 | H99 ΔtatAo::trpA+ | This study |
| KD6 | H99 ΔtatAt::trpA+ containing pKD5 | This study |
| KD7 | H99 ΔtatCo::trpA+ containing pKD8 | This study |
| KD10 | H99 containing pKD10 | This study |
| KD11 | H99 containing pKD11 | This study |
| KD12 | KD5 containing pKD10 | This study |
| KD15 | H99 containing pKD15 | This study |
| KD20 | H99 ΔtatAt::trpA+ containing pKD6 | This study |
| KD21 | H99 ΔtatCo::trpA+ containing pKD7 | This study |
| KD22 | H99 ΔtatCt::trpA+ containing pKD8 | This study |