FIG. 4.

RT-PCR comparing flagellar gene expression in isogenic AlgT⁺ and AlgT⁻ P. aeruginosa strains. RT-PCR was performed on representatives of the four classes of flagellar genes to compare their mRNA levels in isogenic AlgT⁺ and AlgT⁻ P. aeruginosa strains. The RT-PCRs were separated on an agarose gel, and band intensities were compared using a Kodak Image Station 2000RT system. omlA was included as constitutive control (34). Shown are the averages of four independent experiments and standard deviations. Differences (n-fold) in mRNA levels for both microarray and RT-PCRs are indicated.