TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Sinorhizobium meliloti | ||
| 1021 | expR102::ISRm2011-1 expR Smr | 19 |
| 8530 | 1021 expR+ (formerly expR101) Smr | 55 |
| MG32 | 8530 with in-frame sinI deletion (sinI expR+) Smr | This study |
| MG75 | 1021 with in-frame sinI deletion (sinI expR) Smr | This study |
| Escherichia coli | ||
| DH5α | hsdR17 endA1 thi-1 gyrA96 relA1 supE44 ΔlacU169 (φ80dlacZΔM15) | Invitrogen |
| DH10B | F′ mcrA (mrr-hsdRMS-mrcBC) φ80lacZDM15 lacX74 deoR recA1 endA1 araD139Δ(ara leu)7697 galU galK1 | Gibco BRL |
| AHL reporters | ||
| Agrobacterium tumefaciens NTI(pZLR4) | TraR receptor, cognate AHL = 3-oxo-C8-HSL Gmr | 63 |
| Pseudomonas putida 117(pAS-C8) | CepR receptor; cognate AHL = C8-HSL Gmr | 65 |
| E. coli JM109(pSB1075) | LasR receptor; cognate = 3-oxo-C12-HSL Apr | 78 |
| Plasmids | ||
| pBluescript II SK(+/−) | Cloning vector; ColE1 origin Apr | Stratagene |
| pTH113 | Vector pRK7813; intact sinI gene Tcr | 14 |
| pJQ200SK | Cloning vector; p15A origin sacB+ Gmr | 56 |
| pRK600 | pRK2013 Nm::Tn9 Cmr | 51 |
| pVO155 | pUC119-derived integrational vector | 51 |
| pVMG | pVO155 with stop codons upstream of gus in all ORFs | This study |
| pMG1 | Internal 649 bp of Smb21121 (ivdI) in pVMG | This study |
| pMG2 | Internal 354 bp of Smb21133 in pVMG | This study |
| pMG3 | Internal 413 bp of Smc00154 (gor) in pVMG | This study |
| pMG4 | Internal 456 bp of Smc00242 in pVMG | This study |
| pMG5 | Internal 472 bp of Smc01907 in pVMG | This study |
| pMG6 | Internal 354 bp of Smc01966 in pVMG | This study |
| pMG8 | Internal 354 bp of Smc02562 (pckA) in pVMG | This study |
| pMG9 | Internal 472 bp of Smc02689 in pVMG | This study |
| pMG10 | Internal 295 bp of Smc03930 (soxG2) in pVMG | This study |
| pMG11 | Internal 472 bp of Smc40330 (mttB) in pVMG | This study |