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. 2005 Dec;187(23):7931–7944. doi: 10.1128/JB.187.23.7931-7944.2005

TABLE 2.

Identification of sinI- and expR-dependent, AHL-responsive proteins in S. meliloti

Spot no.a Geneb Predicted protein Accumulation dependence Mascot search results
Mol wt/pI
Protein accumulation (% of 8530 expR strain)c
Score No. of peptides matched % of sequence covered Experimental Theoretical sinI sinI + C14 sinI + C16:1 expR sinI expR
52 SMa1296 AdhA1 (EC 1.1.1.1), alcohol degydrogenase expR only 79 9 38 38,794/6.09 36,212/5.68 —d — 140 60 —
23 SMa1450 Probable thiolase sinI, expR 66 16 47 40,346/6.37 40,823/5.87 70 60 80 60 70
50 SMa1993 Conserved hypothetical protein AHLs only 55 4 27 25,370/5.66 24,881/5.65 — 450 690 — —
16 SMa2037 Putative oxidoreductase expR only 66 5 10 88,764/6.71 86,608/8.15 — — — 60 50
22 SMa2041 Probable oxidoreductase sinI, expR 76 8 36 29,565/6.55 29,589/6.05 80 80 80 50 60
18 SMb20170 AdhC3 (EC 1.2.1.1), glutathione-dependent formaldehyde dehydrogenase sinI, expR 74 14 42 40,398/6.56 39,834/6.01 60 70 120 20 30
49 SMb20712 MocB, putative rhizopine uptake ABC transporter periplasmic solute-binding protein precursor sinI only 55 9 33 33,311/4.40 32,849/4.54 180 130 70 — —
38 SMb20899 IdhA (EC 1.1.1.18), myo-inositol dehydrogenase sinI, expR 57 7 31 37,361/5.55 35,125/5.38 170 210 — 240 260
24 SMb21121 IvdH (EC 1.3.99.10), putative isovaleryl-CoAe dehydrogenase expR only 66 22 57 40,274/5.96 41,188/5.58 — — — 50 70
53 SMb21133 Putative sulfate uptake ABC transporter periplasmic solute-binding protein precursor AHLs only 65 7 26 38,333/5.06 37,055/4.97 — 590 610 — —
14 SMb21309 ExpE6, putative membrane protein sinI, expR 94 10 22 46,468/6.14 79,356/5.98 Missing Missing — Missing Missing
20 SMb21321 ExpA4, putative membrane-anchored protein sinI, expR 119 10 37 42,977/6.48 43,684/6.03 20 20 60 20 20
30 SMb21652 LacE, probable lactose uptake ABC transporter periplasmic solute-binding protein precursor sinI, expR 86 8 32 42,005/4.43 45,830/4.50 50 30 40 Missing Missing
35 SMc00153 Conserved hypothetical protein expR only 51 4 41 15,521/4.93 18,912/5.49 — — — 170 160
57 SMc00154 Gor (EC 1.6.4.2), glutathione reductase expR only 55 10 26 45,365/6.39 49,891/5.81 — — — 150 180
27 SMc00242 Signal peptide hypothetical/global homology expR only 74 7 29 39,128/5.16 38,504/5.23 — — — 220 190
39 SMc00361 Hypothetical protein expR only 92 8 45 34,061/5.55 30,703/5.26 — 170 — 170 230
37 SMc00365 PheS (EC 6.1.1.20), phenylalanyl-tRNA synthetase alpha chain sinI, expR 67 7 52 40,532/5.39 40,199/5.13 130 — — 260 290
41 SMc00576 Conserved hypothetical protein expR only 61 7 25 30,675/5.47 32,384/5.18 — — — 150 —
45 SMc00594 LigE, beta-etherase (beta-aryl ether cleaving enzyme) sinI, expR 59 4 32 26,947/6.64 25,486/6.17 40 30 60 50 50
46 SMc00777 Conserved hypothetical protein expR only 73 6 43 16,025/6.48 15,910/5.97 — — — 350 340
40 SMc00910 Conserved hypothetical protein sinI, expR 54 5 27 31,859/5.59 30,503/5.22 170 — — 170 170
13 SMc01345 AccC (EC 6.3.4.14), biotin carboxylase expR only 96 12 43 46,569/6.14 48,968/5.72 — — — 60 —
8 SMc01885 Putative aminopeptidase P sinI, expR 80 9 28 61,931/5.71 65,974/5.38 80 — — 60 60
1 SMc01907 Transmembrane hypothetical/partial homology sinI, expR     double 258 22 51 81,536/4.51 77,127/4.51 — 150 70 — 210
7 SMc01920 NuoG1 (EC 1.6.5.3), NADH dehydrogenase 1 chain G sinI, expR 104 12 26 69,553/5.55 73,962/5.26 New New New New New
54 SMc01966 Putative spermidine/putrescine-binding periplasmic ABC transporter protein sinI, expR 57 7 33 36,728/5.12 39,366/5.33 190 150 — 150 —
25 SMc02047 GcvT (EC 2.1.2.10), aminomethyl transferase (glycine cleavage system protein) expR only 59 16 40 40,241/5.79 40,344/5.41 — — 80 200 210
12 SMc02049 GcvP (EC 1.4.4.2), glycine dehydrogenase decarboxylating expR only 64 14 21 101,185/6.13 104,039/5.66 — — — 260 360
26 SMc02094 Putative tansmembrane outer membrane protein sinI, expR 63 7 14 44,303/5.24 84,469/4.72 160 140 — 220 170
36 SMc02156 Conserved hypothetical protein sinI, expR 55 4 32 31,453/4.66 28,535/4.80 280 240 230 190
4 SMc02365 DegPI (EC 3.4.21.−), protease precursor sinI, expR 166 15 45 58,352/4.86 53,003/4.80 160 160 — 180 180
32 SMc02378 Periplasmic binding protein sinI, expR 141 13 46 35,010/4.59 35,789/4.68 230 210 — 230 170
56 SMc02465 SdhA (EC 1.3.99.1), succinate dehydrogenase flavoprotein subunit sinI expR     double 54 9 21 63,363/6.05 66,935/5.76 — — — — 170
9 SMc02562 PckA (EC 4.1.1.49), phosphoenolpyruvate carboxykinase sinI, expR 140 15 44 53,328/5.92 58,083/5.56 40 20 — 20 20
11 SMc02562 PckA (EC 4.1.1.49), phosphoenolpyruvate carboxykinase sinI, expR 112 16 47 54,049/6.02 58,083/5.56 30 20 — 20 20
55 SMc02562 PckA (EC 4.1.1.49), phosphoenolpyruvate carboxykinase sinI, expR 59 9 24 54,523/5.81 58,083/5.56 40 20 — 20 30
3 SMc02634 Transmembrane hypothetical sinI only 68 10 23 67,942/4.76 73,515/4.78 120 — 140 — —
51 SMc02689 Probable aldehyde dehydrogenase AHLs only 87 14 43 47,617/5.82 55,216/5.43 — 130 190 — —
5 SMc02857 DnaK, heat shock protein 70 (Hsp70) chaperone sinI, expR 51 7 15 65,402/5.14 68,907/4.91 140 200 130 170 190
6 SMc02857 DnaK, heat shock protein 70 (Hsp70) chaperone sinI, expR 54 9 17 65,254/5.11 68,907/4.91 160 220 130 200 140
48 SMc02896 IlvEI (EC 2.6.1.42), branched-chain amino acid aminotransferase sinI only 95 9 33 38,794/5.78 39,587/5.43 190 240 — — 270
21 SMc02898 KdsB (EC 2.7.7.38), 3-deoxy-manno-octulosonate cytidylyltransferase sinI only 81 6 42 31,019/6.87 27,086/6.18 150 140 — — —
28 SMc03061 AglE, ABC transporter α-glucoside-binding periplasmic protein sinI, expR 159 12 41 41,881/4.68 49,672/4.71 170 150 — 160 150
2 SMc03233 Transmembrane hypothetical sinI, expR 111 8 32 70,786/4.57 45,017/4.53 140 140 — 150 120
34 SMc03786 Bfr, bacterioferritin expR only 69 5 56 25,678/4.89 18,337/4.75 — — — 160 160
59 SMc03823 LeuC (EC 4.2.1.33), 3-isopropylmalate dehydratase large subunit expR only 67 11 31 48,110/6.03 50,963/5.58 — — — 180 170
33 SMc03864 ABC transporter amino acid-binding periplasmic protein sinI, expR 111 11 44 26,329/4.47 29,353/4.65 220 210 140 250 210
44 SMc03930 SoxG2 (EC 1.5.3.1), sarcosine oxidase gamma subunit expR only 56 5 39 30,035/6.46 18,917/5.78 — — — 40 40
29 SMc03969 Conserved hypothetical protein AHLs only 70 6 35 29,861/5.05 26,729/4.81 — 190 170 — —
47 SMc03972 MexE2, transmembrane multidrug efflux system sinI, expR 55 4 15 40,359/5.22 41,853/5.46 30 40 80 30 60
17 SMc03983 FbaB (EC 4.1.2.13), fructose-bisphosphate aldolase class I sinI, expR 112 14 43 39,531/6.83 37,297/6.22 50 30 — 40 30
19 SMc03983 FbaB (EC 4.1.2.13), fructose-bisphosphate aldolase class I sinI, expR 96 11 41 39,599/6.58 37,023/6.22 50 40 — 40 50
58 SMc04040 IbpA, heat shock protein expR only 77 8 53 29,852/6.58 17,402/6.11 — — — 70 180
10 SMc04330 MttB, trimethylamine methyltransferase expR only 50 10 27 49,259/5.85 56,934/5.35 — — — 30 50
15 SMc04347 Conserved hypothetical protein expR only 53 17 33 81,117/6.51 73,870/6.11 — — — Missing Missing
31 No good match sinI, expR 40,333/4.40 New New — New New
42 No good match sinI, expR 16,294/5.02 80 80 90 60 40
43 No good match sinI, expR 50,866/5.71 190 210 — 400 370
a

Numbers refer to protein spots in Fig. 1.

b

Bolded genes were tested as single-crossover fusions (see Table 4).

c

Averages from two independent cultures and four independent gels per treatment or strain.

d

Value not significantly different from accumulation in 8530.

e

CoA, coenzyme A.