TABLE 3.
Activity of single-crossover gus fusions in genes corresponding to QS-regulated proteins
| Strain background for single crossover | GUS activity (Miller units)a [culture OD]b with the indicated protein accumulation dependence:
|
||||||
|---|---|---|---|---|---|---|---|
| ivdH, Smb21121 (expR only)c | SO4 transporter, Smb21133 (AHLs only) | Hypothetical signal peptide, Smc00242 (expR only) | pckA, Smc02562 (sinI, expR) | Aldehyde dehydrogenase, Smc02689 (AHLs only) | soxG2 Smc03930 (expR only) | mttB, Smc04330 (expR only) | |
| WT | 26† [0.7 ± 0.1] | 1.4 × 102† [0.7 ± 0.1] | 4.2 × 102† [0.8 ± 0.1] | 3.9 × 103† [0.6 ± 0.1] | 4.5 × 102† [0.6 ± 0.1] | 18† [0.8 ± 0.1] | 3.3 × 103† [1.3 ± 0.2] |
| expR | 21‡ | 2.0 × 102‡ | 5.7 × 102‡ | 0.6 × 103‡ | 3.3 × 102‡ | 3.5‡ | 1.0 × 103‡ |
| sinI expR | 21‡ | 2.1 × 102‡ | 5.7 × 102‡ | 0.8 × 103‡ | 3.0 × 102‡ | 3.1‡ | NAd† |
| sinI | 18§ | 1.7 × 102† | 5.4 × 102‡ | 0.9 × 103‡ | 2.9 × 102‡ | 2.9‡ | 3.2 × 103† |
| sinI + C14 | 16§ | 1.9 × 102‡ | 4.6 × 102†§ | 0.7 × 103‡ | 3.2 × 102‡ | 6.3‡ | 3.1 × 103† |
| sinI + C16:1 | 18§ | 1.9 × 102‡ | 3.9 × 102† | 3.0 × 103§ | 3.8 × 102§ | 4.4‡ | 3.4 × 103† |
| WT | 12 × 102† [1.8 ± 0.1] | 4.8 × 102† [1.8 ± 0.1] | 2.2 × 103† [1.8 ± 0.1] | 25 × 103† [1.8 ± 0.1] | 1.8 × 103† [2.0 ± 0.1] | 520† [2.0 ± 0.1] | 4.7 × 103† [2.0 ± 0.1] |
| expR | 10 × 102† | 9.0 × 102‡ | 6.2 × 103‡ | 20 × 103‡ | 2.9 × 103† | 23‡ | 2.0 × 103‡ |
| sinI expR | 10 × 102† | 8.6 × 102‡ | 6.2 × 103‡ | 21 × 103‡ | 2.5 × 103† | 22‡ | NA§ |
| sinI | 8.5 × 102† | 7.7 × 102‡ | 6.2 × 103‡ | 19 × 103‡ | 2.2 × 103† | 17‡ | 5.6 × 103§ |
| sinI + C14 | NA† | 7.4 × 102‡ | 3.5 × 103† | 23 × 103†‡§ | 4.2 × 103‡ | 18‡ | 5.3 × 103§ |
| sinI + C16:1 | 8.1 × 102† | 5.9 × 102† | 5.9 × 103‡ | 26 × 103§ | 4.8 × 103‡ | 19‡ | 5.3 × 103§ |
Values are averages from three replicate cultures for each test strain in a representative experiment. Values followed by different symbols are significantly different from each other at the 0.05 level according to Tukey's studentized range (HSD) test.
OD values apply to all values in the section of the column in which they appear.
Protein accumulation dependence is indicated in Table 2. Fusions in Smc00154, Smc01907, and Smc01966 were tested similarly to those shown and had no significant changes in activity.
NA, value not available from this experiment. The value from an independent experiment was not significantly different from the value for the sinI mutant.