TABLE 2.
Selected list of Erwinia amylovora genes induced during infection of immature pear tissuea
Function | pfi no. | Geneb | Organism | Blastx_BestHitc | GenBank accession no. | Previously reportedd |
---|---|---|---|---|---|---|
TTSS and effectors | 1 | dspE* | Erwinia amylovora | DspE/pathogenicity factor | AAC04850 | Ech, 77 |
2 | hrpGF | E. amylovora | Type III secretion HrpG/F | AAB49178 | Pst, 12 | |
3 | hrpL* | E. amylovora | Type III sensor kinase HrpL | AAD24682 | ||
4 | hrpX* | E. amylovora | Type III secretion HrpX | AAD24681 | ||
5 | hopPtoC | Pseudomonas syringae pv. tomato | Type III effector HopPtoC | NP_790436 | Pst, 12 | |
GSP | 6 | secA* | Salmonella enterica serovar Typhimurium | Preprotein translocase SecA | NP_459141 | |
7 | secDF* | Yersinia pestis | Protein export membrane protein SecDF | NP_406663 | Apl, 47 | |
8 | secB* | Escherichia coli | Protein export protein SecB | NP_756294 | Ppu, 60 | |
T2SS and proteins secreted by T2SS | 9 | yts11J | Yersinia enterocolitica | Type II secretion Yts1IJ protein | CAC83035 | Rso, Ech; 16, 77 |
10 | lsc* | E. amylovora | Levansucrase | CAA52972 | ||
11 | peh | Pectobacterium carotovorum | Peh (polygalacturonase) | BAA74431 | Pst, 12 | |
Proteins known to be secreted by other secretion systems | 12 | fhaB* | Escherichia coli | Putative adhesin/hemagglutinin/hemolysin | AAQ19127 | Rso, 16 |
13 | hecA | Erwinia chrysanthemi | Hemolysin/hemagglutinin-like protein HecA | AAN38708 | Rso, 16 | |
14 | mltE | Yersinia pestis | Membrane-bound lytic murein transglycosylase | NP_405972 | Pst, 12 | |
15 | prtA* | Erwinia amylovora | Zinc-binding metalloprotease | CAB42873 | ||
General, ion, sugar, amino acid, and nucleotide transport | 16 | abc | Yersinia pestis | ABC transporter ATP-binding protein (inorganic ion transport) | NP_404687 | Ech, 77 |
17 | pstA | Yersinia pestis | Putative phosphate transport system permease | NP_406342 | ||
18 | ppx | Pantoea agglomerans | Exopolyphosphatase | AAQ14878 | Sty, 55 | |
19 | ftn | Yersinia pestis | Cytoplasmic ferritin (an iron storage protein) | NP_669829 | ||
20 | kefB | Salmonella enterica serovar Typhimurium | K+/H+ antiporter | NP_462361 | Rso, 16 | |
21 | c3774* | Escherichia coli | Ferric enterobactin transport ATP-binding protein | NP_755645 | ||
22 | btuB | Serratia marcescens | Outer membrane receptor for iron transport | AAL50647 | ||
23 | Gmet1925 | Geobacter metallireducens | ABC-type nitrate/sulfonate/bicarbonate transport system | ZP_00081179 | ||
24 | exbB | Salmonella enterica | Biopolymer transport ExbB protein | NP_457552 | ||
25 | kgtP* | Salmonella enterica serovar Typhimurium | Alpha-ketoglutarate permease | NP_461589 | Rso, 16 | |
26 | ECs2608 | Escherichia coli | ATP-binding component of high-affinity l-arabinose transport system | NP_310635 | ||
27 | citA | Pseudomonas aeruginosa | Citrate transporter | NP_254163 | ||
28 | rbsD | Yersinia pestis | High-affinity d-ribose permease | NP_403673 | ||
29 | gntU | Salmonella enterica serovar Typhimurium | Low-affinity gluconate permease | NP_462442 | ||
30 | mglA* | Salmonella enterica serovar Typhimurium | Methylgalactoside transport protein | NP_461134 | ||
31 | emrB | Escherichia coli | Multidrug resistance protein B | NP_755121 | Rso/Eco; 16, 68 | |
32 | mdl | Klebsiella pneumoniae | Multidrug resistance-like ATP-binding protein | CAA07091 | ||
33 | putP* | Salmonella sp. | Proline permease | AAA99282 | ||
34 | Avin4550* | Azotobacter vinelandii | Permeases of the major facilitator superfamily | ZP_00092807 | Pfl, 67 | |
35 | rbsB | Streptococcus agalactiae | Ribose ABC transporter, periplasmic d-ribose-binding protein | NP_687150 | ||
36 | livK | Yersinia pestis | High-affinity leucine-specific-binding periplasmic protein; branched-chain amino acid ABC transport system | NP_667760 | ||
37 | ECs3191 | Escherichia coli | Histidine transport system membrane protein M | NP_311218 | ||
38 | lysP | Shigella flexneri | Lysine-specific permease | NP_708053 | ||
39 | YPO3257* | Yersinia pestis | Amino acid ABC transporter, periplasmic protein | NP_406727 | Rso, 16 | |
40 | metC* | E. coli | Beta-cystathionase | AAA69175 | ||
41 | lysC | Yersinia pestis | Lysine-sensitive aspartokinase III | NP_407170 | ||
42 | avtA* | Escherichia coli | Valine-pyruvate aminotransferase | NP_756255 | ||
43 | oppA | Yersinia pestis | Oligopeptide ABC transporter; periplasmic binding protein | NP_669341 | ||
44 | oppC | Yersinia pestis | Oligopeptide transport system permease protein | NP_405727 | ||
45 | tsx | Salmonella | Nucleoside-specific channel-forming protein | NP_455008 | ||
46 | hemH | Yersinia enterocolitica | Ferrochelatase | AAC60760 | ||
47 | uup | Salmonella enterica serovar Typhimurium | Putative ABC transporter ATPase component | NP_460036 | Rso, 16 | |
48 | sbmA | Salmonella enterica serovar Typhimurium | Putative ABC transporter membrane protein | NP_459371 | Rso, 16 | |
49 | y0619* | Yersinia pestis | Putative periplasmic binding transport protein | AAM84207 | ||
50 | hemT | Yersinia enterocolitica | Hemin binding protein (periplasm binding protein-hemin transport) | CAA54866 | ||
51 | yadH* | Salmonella enterica serovar Typhimurium | Putative transport protein | NP_459178 | ||
Bacterial cell surface and transmembrane | 52 | nlpA* | Escherichia coli | Lipoprotein 28 | NP_418117 | Rso, 16 |
53 | dacB | Yersinia pestis | d-Alanyl-d-alanine carboxypeptidase/penicillin-binding protein 4 | NP_668015 | Rso, 16 | |
54 | kpsC | Escherichia coli | Capsule polysaccharide export protein | P42217 | Eco, 68 | |
55 | dinF | Salmonella enterica | DNA damage-inducible membrane protein | NP_458536 | Rso, 16 | |
56 | corB* | Salmonella enterica | Putative membrane protein | NP_457149 | Ech, 77 | |
57 | ytfK* | Salmonella enterica serovar Typhimurium | Putative cytoplasmic protein | NP_463267 | Ech, 77 | |
58 | YPO2305 | Yersinia pestis | Putative exported protein | NP_405842 | ||
59 | tolA | Salmonella enterica serovar Typhimurium | Tol import system inner membrane protein | NP_459732 | ||
60 | tolR | Erwinia chrysanthemi | TolR protein inner membrane protein interacting with TolA and TolQ | CAC82707 | ||
61 | matE* | Salmonella enterica | Putative inner membrane protein | NP_456567 | Rso, 16 | |
62 | STM0278* | Salmonella enterica serovar Typhimurium | Putative periplasmic protein | NP_459276 | Rso, 16 | |
63 | ydiY | Salmonella enterica serovar Typhimurium | Putative outer membrane protein (salt inducible) | NP_460293 | ||
64 | ompC | Escherichia coli | Outer membrane protein C (porin OmpC) | Q54471 | ||
65 | ompA* | Salmonella enterica | Putative outer membrane protein (OmpA) | NP_458280 | ||
66 | Z1931* | Escherichia coli | Outer membrane protein 3b (a), protease VII | NP_287408 | ||
67 | ptrA* | Yersinia pestis | Protease III precursor | NP_404633 | ||
68 | mrdB | Escherichia coli | Rod shape-determining protein RodA | NP_752655 | ||
69 | flgN | Shigella flexneri | Flagellum synthesis protein FlgN | NP_836779 | ||
70 | fliG | Shigella flexneri | Flagellar motor switch protein FliG | NP_707824 | ||
71 | fliM | Escherichia coli | Flagellar motor switch protein FliM | NP_754254 | ||
72 | ycgB | Escherichia coli | Putative sporulation protein | NP_287427 | ||
Mobile elements-phage | 73 | STY4666* | Salmonella enterica | Probable phage integrase | NP_458745 | |
74 | intD | Escherichia coli | Prophage DLP12 integrase | NP_415069 | ||
Stress response | 75 | mutS | Yersinia pestis | DNA mismatch repair protein MutS | NP_406817 | Sen, 33 |
76 | recA | Yersinia pestis | RecA protein | NP_406773 | Sau, 47 | |
77 | sulA | Enterobacter aerogenes | Cell division inhibitor, SOS regulon gene | C29016 | ||
78 | dps | Serratia marcescens | DNA protection during starvation protein (Dsp) | AAO47741 | ||
79 | cstA | Salmonella enterica | Carbon starvation protein | NP_458955 | ||
80 | ycbP* | Escherichia coli | Sulfate starvation-induced protein 4/FMNe reductase | NP_753005 | ||
81 | ahpC* | Photorhabdus luminescens | Alkyl hydroperoxide reductase, small subunit (antioxidant) | NP_931108 | ||
82 | cyoB* | Escherichia coli | Cytochrome o ubiquinol oxidase subunit I | NP_286173 | ||
83 | ydiJ | Salmonella enterica serovar Typhimurium | Putative oxidase | NP_460330 | ||
84 | bacA | Salmonella enterica serovar Typhimurium | Bacitracin resistance protein | NP_462120 | Ech, 77 | |
85 | pqiB | Yersinia pestis | Paraquat-inducible protein B | NP_670048 | ||
86 | grpE | Photorhabdus luminescens | GrpE protein (HSP-70 cofactor) (heat shock protein B25.3) | NP_930590 | Rso, 16 | |
87 | y1165 | Yersinia pestis | Putative cold shock protein | NP_668491 | ||
88 | pspB* | Yersinia enterocolitica | Phage shock protein B | AAG22114 | ||
89 | pspC | Yersinia enterocolitica | Phage shock protein C | AAG22115 | ||
90 | tsr* | Salmonella enterica serovar Typhimurium | Methyl-accepting chemotaxis protein I | NP_463392 | Ech, 77 | |
Regulation | 91 | rlsA | Erwinia amylovora | RlsA protein/LysR homolog | CAA10420 | |
92 | rcsA* | Erwinia amylovora | Colanic acid capsular biosynthesis activation protein A | A45828 | ||
93 | grrS* | Serratia plymuthica | Putative global response regulation sensor kinase | AAL11449 | ||
94 | envZ* | Yersinia pestis | Histidine kinase/EnvZ/osmolarity sensor protein | NP_403793 | Sty, 33 | |
95 | fliZ | Yersinia pestis | Putative alternative sigma factor regulatory protein | NP_405408 | ||
96 | gntR | Salmonella enterica | Gluconate utilization operon repressor | NP_458376 | ||
97 | deoR | Salmonella enterica | DeoR/deoxyribose operon repressor | NP_455391 | ||
98 | metJ | Photorhabdus luminescens | Met repressor (Met regulon regulatory protein MetJ)/repressor of the methionine regulon | NP_931917 | ||
99 | hlfK | Shigella flexneri | Protease specific for phage lambda cII repressor | NP_710039 | ||
100 | yqhC* | Escherichia coli | Putative AraC-type regulatory protein | NP_289587 | ||
101 | ybiQ | Shigella flexneri | Transcriptional regulator MntR | NP_706694 | ||
102 | budR | Raoultella terrigena | HTH-type transcriptional regulator | P52666 | ||
103 | c5058* | Escherichia coli | Putative transcriptional regulator LysR | NP_756910 | Ech, 77 | |
104 | PSPTO3576 | Pseudomonas syringae pv. tomato | TetR family transcriptional regulator | NP_793355 | ||
105 | YPO3913* | Yersinia pestis | TetR family transcriptional regulatory protein | NP_407358 | ||
106 | YPO0736 | Yersinia pestis | Putative regulatory protein | NP_404367 | ||
107 | YPO0315* | Yersinia pestis | Regulatory protein (multiple antibiotic resistance) | NP_403966 | ||
108 | fnr | Escherichia coli | Fumarate and nitrate reduction regulatory protein | NP_753709 | ||
109 | glnK | Yersinia pestis | Nitrogen regulatory protein P-II | NP_406618 | ||
110 | modE | Escherichia coli | Molybdate uptake regulatory protein | NP_286482 | ||
111 | pspF | Salmonella enterica | Psp operon transcriptional activator | NP_455812 | ||
112 | hlyB | Yersinia pestis | Putative hemolysin activator protein | NP_406025 | ||
113 | rbsR* | Shigella flexneri | Regulator for rbs operon | NP_839112 | ||
114 | Psyr0512 | Pseudomonas syringae | Rhs family protein | ZP_00124230 | ||
115 | STY4601* | Salmonella enterica | Putative regulator of late gene expression | NP_458684 | ||
116 | kdpE | Salmonella enterica serovar Typhimurium | Response regulator in two-component regulatory system with KdpD | AAL19646 | ||
117 | PA2177 | Pseudomonas aeruginosa | Probable sensor/response regulator hybrid | NP_250867 | ||
118 | y3531* | Yersinia pestis | Putative kinase | NP_670828 | ||
119 | YPO0014 | Yersinia pestis | Putative type II homoserine kinase/YihE | NP_403681 | ||
120 | Avin4532 | Azotobacter vinelandii | Serine/threonine protein kinase | ZP_00092789 | ||
121 | Bcep3208 | Burkholderia fungorum | Sugar kinases, ribokinase family | ZP_00030390 | ||
122 | NE2503 | Nitrosomonas europaea | TonB-dependent receptor protein | NP_842492 | ||
123 | hrpA | Escherichia coli | ATP-dependent helicase HrpA | P43329 | Rso, 16 | |
124 | dbpA* | Yersinia pestis | ATP-dependent RNA helicase | NP_405343 | ||
125 | rpoN | Klebsiella pneumoniae | RNA polymerase sigma-54 factor | P06223 | Rso, 16 | |
126 | rpoD | Pantoea agglomerans | RNA polymerase sigma-70 subunit | AAL11450 | ||
Metabolism and nutrient scavenging | 127 | ilvI* | Yersinia pestis | Acetolactate synthase III | NP_670937 | Pst, 12 |
128 | purF | Escherichia coli | Amidophosphoribosyltransferase | NP_288886 | Rso, 16 | |
129 | argG* | Photorhabdus luminescens | Argininosuccinate synthase | NP_931904 | Rso, 16 | |
130 | pheA | Erwinia herbicola | Chorismate mutase/prephenate dehydratase | S26053 | Ech, 77 | |
131 | hemB | Salmonella enterica | Delta-aminolevulinic acid dehydratase | NP_454967 | Rso, 16 | |
132 | glgX | Escherichia coli | Glucose-1-phosphate adenylyltransferase | NP_756082 | Rso, 16 | |
133 | murB* | Escherichia coli | Oxidoreductase/UDP-N-acetylpyruvoylglucosamine reductase | AAA24185 | Rso/Pst; 16,12 | |
134 | pksC* | Mycobacterium leprae | Polyketide synthase PksC | S73013 | Rso, 16 | |
135 | YPO2195 | Yersinia pestis | Putative acyl coenzyme A thioester hydrolase | NP_405738 | Pst, 12 | |
136 | ygfZ | Salmonella enterica serovar Typhimurium | Putative aminomethyltransferase | NP_461964 | Pst, 12 | |
137 | YPO2310 | Yersinia pestis | Putative carboxypeptidase | NP_405847 | Pst, 12 | |
138 | truD | Yersinia pestis | Putative hydrogenase subunit | AAM84415 | Pst, 12 | |
139 | aceE | Salmonella enterica | Pyruvate dehydrogenase E1 component | NP_454766 | Pst, 12 | |
140 | rhsD | Yersinia pestis | RhsD protein | NP_667608 | ||
141 | hemD | Salmonella enterica serovar Typhimurium | Uroporphyrinogen III synthase | NP_462823 | ||
142 | pyrG* | Salmonella enterica | CTP synthase (UTP-ammonia ligase) | NP_457342 | Rso, 16 | |
143 | dnaQ* | Escherichia coli | DNA polymerase III, epsilon chain | NP_752198 | Rso, 16 | |
144 | argA | Yersinia pestis | Amino acid acetyltransferase | NP_404636 | Vch, 50 | |
145 | pepN* | Salmonella enterica serovar Typhimurium | Aminopeptidase N | NP_460031 | Rso, 16 | |
146 | glnA* | Yersinia pestis | Glutamine synthetase | NP_671098 | Rso/Eco, 16,68 | |
147 | cheR | Yersinia pestis | Glutamate methyltransferase | NP_669155 | ||
148 | moaC* | Salmonella enterica | Molybdenum cofactor biosynthesis protein C | NP_455345 | Pfl, 67 | |
149 | moeC | Yersinia pestis | Molybdopterin biosynthesis protein | NP_669976 | ||
150 | YPO2420* | Yersinia pestis | Probable formyl transferase | NP_405953 | ||
151 | YPO2174* | Yersinia pestis | Putative nucleotide sugar dehydrogenase | NP_405718 | ||
152 | YPO1009 | Yersinia pestis | Probable peptidase (PepT) | NP_286807 | ||
153 | fbp | Escherichia coli | Fructose-1,6-bisphosphatase | NP_757176 | ||
154 | gnd | Yersinia pestis | Gluconate-6-phosphate dehydrogenase | NP_669932 | ||
155 | zwf | Yersinia pestis | Glucose-6-phosphate dehydrogenase | NP_405618 | ||
156 | pckA | Salmonella enterica serovar Typhimurium | Phosphoenolpyruvate carboxykinase | NP_462403 | ||
157 | ECs0188 | Escherichia coli | Lysine decarboxylase 2 | NP_308215 |
Hypothetical and unknown genes are not listed.
Gene name designations were based on originally reported gene products that shared high similarity to pfi clone sequences. Asterisks after the gene name indicate that two or more clones were identified for the same gene in the experiment.
Predicted proteins or functions based on similar proteins identified using BlastX searches.
Genes identified to be induced in other bacterium plant or animal systems using a similar IVET screen or other in vivo expression systems. Rso, Ralstonia solanacearum; Ech, Erwinia chrysanthemi; Pst, Pseudomonas syringae pv. tomato; Pfl, Pseudomonas fluorescens; Ppu, Pseudomonas putida; Sen, Salmonella enterica; Sty, Salmonella enterica serovar Typhimurium; Sau, Staphylococcus aureus; Apl, Actinobacillus pleuropneumoniae; Vch, Vibrio cholerae; Eco, Escherichia coli. Numbers indicate the references.
FMN, flavin mononucleotide.