TABLE 3.
Expression of IVET clones after inoculation of immature pear fruit
| pfi clone or strain | Gene homolog | 0 hpia
|
24 hpi
|
48 hpi
|
||
|---|---|---|---|---|---|---|
| GUS activityb | GUS activity | Fold inductionc | GUS activity | Fold induction | ||
| pZYF2 | dspErevd | ULf | UL | 0 | UL | 0 |
| pZYF8 | dspEfore | 1.0 ± 2.1 | 282.0 ± 145.5 | 282.0 | 118.3 ± 18.4 | 118.3 |
| pfi5 | hopPtoC | 10.8 ± 6.7 | 11.8 ± 4.4 | 1.1 | 90.1 ± 43.3 | 8.4 |
| pfi9 | yts11J | 37.1 ± 2.0 | 74.7 ± 34.9 | 2.0 | 128.0 ± 48.8 | 3.5 |
| pfi43 | oppA | 1.0 ± 0.9 | 91.4 ± 27.4 | 91.4 | 138.9 ± 25.2 | 138.9 |
| pfi91 | rlsA | 1.0 ± 1.3 | UL | 0 | 19.2 ± 12.6 | 19.2 |
| pfi93 | grrS | 77.7 ± 2.3 | 30.2 ± 7.0 | 0.4 | 106.3 ± 35.8 | 1.4 |
| sav2932g | 107.4 ± 3.5 | 852.2 ± 380.3 | 7.9 | 86.2 ± 40.4 | 0.8 | |
a
Hours postinoculation (hpi).
b
GUS activity is shown in μmol of 4-methylumbelliferyl produced min−1 109 CFU−1. Data represent the means of three measurements ± standard errors. Similar results were obtained in a second independent experiment.
c
Fold induction is shown as GUS activity at 24 or 48 hpi/GUS activity at 0 hpi.
d
dspE promoter in opposite direction from uidA gene.
e
dspE promoter in correct direction toward uidA gene.
f
UL, under detection limit of vector control.
g
sav2932 encodes a hypothetical protein and is not listed in Table 2.