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. 2005 Dec;187(23):7901–7911. doi: 10.1128/JB.187.23.7901-7911.2005

FIG. 6.

FIG. 6.

Increased excision of ST64B does not contribute to the phenotype of envelope instability of dam mutants. Protein extracts were prepared from culture supernatants of the following isogenic strains: SL1344, wild type; SV1610, dam; MD1104, Δ[ST64B]; MD1202, Δ[ST64B] dam; MA6247, Δ[Gifsy-1 Gifsy-2]; MD1203, Δ[Gifsy-1 Gifsy-2] dam; MA7551, Δ[ST64B Gifsy-1 Gifsy-2]; MD1201, Δ[ST64B Gifsy-1 Gifsy-2] dam; MA7891, Δ[ST64B Gifsy-1 Gifsy-2 SopEΦ]; and MD0200, Δ[ST64B Gifsy-1 Gifsy-2 SopEΦ] dam. Bacteria were grown overnight in LB medium at 37°C in static conditions. The amount loaded per lane corresponded to the same number of bacteria (2 × 109). The sizes of the prestained molecular weight markers are indicated in kilodaltons. Arrows highlight a prominent protein that is only observed in dam mutants carrying the ST64B prophage. The assay was repeated three times with similar results.