TABLE 2.

Oligonucleotides used to generate deletions mutants

Oligonucleotidea	Sequence (5′→3′)b	Prophage or gene deleted
sb49-3D	ATGACACCACGTCAACGCCGTCAACATCGTAACGCAATAGAAAAAGCCGCTGTGTAGGCTGGAGCTGCTTC	sb49
sb49-4R	TCAAGATATTTTTTCATTTTGCTTTCTCATTGCATCCCTGAATGCGAGAAAATTCCGGGGATCCGTCGACC	sb49
sb50-1D	ATGCTTGGCAAAGCTGCGCCGCGATGGCAGCGACGAAAAGCAAGTCAATTAGTGTAGGCTGGAGCTGCTTC	sb50
sb50-2R	ACATGCCAGCAGAAGCTGCGCAATTTCTCCGCTCGCTGAAACTGCCAGTCAATTCCGGGGATCCGTCGAC	sb50
pp291	AGAATATCGCGCAGTACCAGTATGTTTCCGCCGTTCATCACAGATCATCGAGCTCTCCCG	ST64B
pp293	ATACCTGAAAGCTGCTTCTGGTATGTGCTGCATGACCGCATGTAGGCTGGAGCTGCTTCG	ST64B
pp339	ACTGTACTTCTGCTTGTCTTTTGCCGTTCCCTCATAGTCTCATATGAATATCCTCCTTAG	SopEΦ
pp340	TTAACTCCCTTCCGGTTAGCCGATAACAGAATCCAGTACATGTAGGCTGGAGCTGCTTCG	SopEΦ

^asb primers were used with template plasmid pKD13 (14); pp291 and pp293 were used with template plasmid pNFB22 (Figueroa-Bossi and Bossi, unpublished results); pp339 and pp340 were used with plasmid pKD4 (14).

^bSequences annealing to the template plasmid pKD13 are in italics.