TABLE 1.
P. aeruginosa proteins with increased abundance during anaerobic growth
| Genea | Protein | Gene name | nb | Ratioc | SD |
|---|---|---|---|---|---|
| PA0025* | Shikimate dehydrogenase | aroE | 3 | 1.79 | 0.04 |
| PA0130 | Probable aldehyde dehydrogenase | 10 | 2.28 | 0.22 | |
| PA0132 | Beta-alanine-pyruvate transaminase | 10 | 1.64 | 0.31 | |
| PA0286 | Probable fatty acid desaturase | 5 | 4.61 | 0.42 | |
| PA0300 | Polyamine transport protein | spuD | 7 | 1.65 | 0.17 |
| PA0321 | Probable acetylpolyamine aminohydrolase | 1 | 1.91 | NAd | |
| PA0336 | Nudix hydrolase YgdP | ygdP | 13 | 1.54 | 0.40 |
| PA0396 | Twitching motility protein PilU | pilU | 8 | 1.88 | 0.25 |
| PA0408 | Twitching motility protein PilG | pilG | 2 | 1.63 | 0.10 |
| PA0413 | Component of signal transduction system | chpA | 12 | 2.10 | 0.35 |
| PA0520 | Regulatory protein NirQ | nirQ | 59 | 2.21 | 0.33 |
| PA0655 | Hypothetical protein | 34 | 2.63 | 0.41 | |
| PA0658 | Probable short-chain dehydrogenase | 1 | 1.96 | NA | |
| PA0844 | Hemolytic phospholipase C precursor | plcH | 1 | 1.72 | NA |
| PA0867 | Hypothetical protein | 4 | 2.33 | 0.12 | |
| PA0934 | GTP pyrophosphokinase | relA | 6 | 1.70 | 0.08 |
| PA0936 | LPS biosynthetic protein LpxO2 | lpxO2 | 14 | 2.17 | 0.35 |
| PA1155 | Ribonucleoside reductase, small chain | nrdB | 3 | 12.15 | 5.64 |
| PA1156 | Ribonucleoside reductase, large chain | nrdA | 4 | 3.57 | 1.37 |
| PA1398 | Hypothetical protein | 1 | 1.56 | NA | |
| PA1566 | Conserved hypothetical protein | 3 | 3.12 | 0.58 | |
| PA1681 | Chorismate synthase | aroC | 5 | 1.65 | 0.14 |
| PA1766 | Hypothetical protein | 3 | 1.60 | 0.13 | |
| PA1847 | Conserved hypothetical protein | 1 | 1.88 | NA | |
| PA1919 | Probable radical-activating enzyme | 5 | 7.34 | 0.98 | |
| PA1920 | Conserved hypothetical protein | 15 | 10.80 | 5.21 | |
| PA2119 | Alcohol dehydrogenase (Zn dependent) | 25 | 1.84 | 0.22 | |
| PA2127 | Conserved hypothetical protein | 6 | 2.46 | 0.12 | |
| PA2323 | Probable glyceraldehyde-3-phosphate dehydrogenase | 2 | 1.95 | NA | |
| PA2567 | Hypothetical protein | 1 | 1.54 | NA | |
| PA2945 | Conserved hypothetical protein | 2 | 2.36 | 0.30 | |
| PA2991 | Soluble pyridine nucleotide transhydrogenase | sth | 20 | 1.93 | 0.37 |
| PA2994 | NA+-translocating NADH:quinone oxidoreductase | nqrF | 15 | 1.60 | 0.27 |
| PA2999* | NA+-translocating NADH:ubiquinone oxidoreductase | nqrA | 5 | 1.74 | 0.11 |
| PA3002 | Transcription-repair coupling protein Mfd | mfd | 2 | 1.52 | 0.06 |
| PA3150 | LPS biosynthesis protein WbpG | wbpG | 1 | 3.72 | NA |
| PA3185 | Hypothetical protein | 4 | 1.82 | 0.08 | |
| PA3391 | Regulatory protein NosR | nosR | 5 | 7.75 | 0.98 |
| PA3392 | Nitrous oxide reductase precursor | nosZ | 69 | 3.65 | 0.72 |
| PA3394 | NosF protein | nosF | 9 | 4.09 | 0.46 |
| PA3438 | GTP cyclohydrolase I precursor | folEI | 1 | 5.58 | NA |
| PA3515 | Hypothetical protein | 1 | 4.21 | NA | |
| PA3562* | Probable phosphotransferase system enzyme I | 3 | 2.91 | 0.17 | |
| PA3694 | Hypothetical protein | 4 | 1.92 | 0.07 | |
| PA3871 | Probable peptidyl-prolyl cis-trans isomerase, PpiC type | 3 | 2.50 | 0.64 | |
| PA3873 | Respiratory nitrate reductase delta chain | narJ | 1 | 3.20 | NA |
| PA3874 | Respiratory nitrate reductase beta chain | narH | 67 | 7.89 | 2.83 |
| PA3875 | Respiratory nitrate reductase alpha chain | narG | 35 | 7.70 | 3.23 |
| PA3880 | Conserved hypothetical protein | 8 | 3.88 | 0.98 | |
| PA3886 | Hypothetical protein | 1 | 7.25 | NA | |
| PA3895 | Probable transcriptional regulator | 2 | 1.49 | 0.00 | |
| PA3913 | Probable protease | 1 | 5.30 | NA | |
| PA3914* | Molybdenum cofactor biosynthetic protein A1 | moeA1 | 21 | 3.41 | 0.63 |
| PA3915* | Molybdopterin biosynthetic protein B1 | moaB1 | 5 | 4.40 | 0.72 |
| PA3918* | Molybdopterin biosynthetic protein C | moaC | 23 | 1.88 | 0.41 |
| PA3958 | Hypothetical protein | 1 | 2.29 | NA | |
| PA4180 | Probable acetolactate synthase large subunit | 2 | 2.16 | 0.53 | |
| PA4811 | Nitrate-inducible formate dehydrogenase, beta subunit | fdnH | 3 | 5.85 | 1.85 |
| PA4812 | Formate dehydrogenase-O, major subunit | fdnG | 4 | 3.46 | 0.64 |
| PA4868 | Urease alpha subunit | ureC | 1 | 1.51 | NA |
| PA4922 | Azurin precursor | azu | 4 | 2.96 | 0.81 |
| PA5005 | Probable carbamoyl transferase | 42 | 1.59 | 0.24 | |
| PA5011 | Heptosyltransferase I | waaC | 4 | 1.49 | 0.17 |
| PA5012 | Heptosyltransferase II | waaF | 6 | 1.45 | 0.11 |
| PA5015 | Pyruvate dehydrogenase | aceE | 111 | 1.98 | 0.42 |
| PA5064 | Hypothetical protein | 1 | 1.93 | NA | |
| PA5223 | UbiH protein | ubiH | 3 | 1.67 | 0.10 |
| PA5296 | ATP-dependent DNA helicase Rep | rep | 2 | 1.77 | 0.00 |
| PA5300 | Cytochrome c5 | cycB | 13 | 1.91 | 0.21 |
| PA5332 | Catabolite repression control protein | crc | 3 | 1.90 | 0.21 |
| PA5440 | Probable peptidase | 1 | 18.54 | NA | |
| PA5496* | Hypothetical protein | 8 | 6.46 | 2.07 | |
| PA5497* | Hypothetical protein | 10 | 11.28 | 3.17 | |
| PA5508 | Probable glutamine synthetase | 11 | 2.73 | 0.26 | |
| PA5564 | Glucose inhibited division protein B | gidB | 2 | 1.53 | 0.02 |
a
Genes marked with an asterisk (*) were identified as up-regulated during anaerobic growth (1).
b
Number of peptides identified and quantified for each protein.
c
Values represent relative protein abundance, or the ratio of protein expression in cells grown anaerobically to protein expression in cells grown aerobically.
d
NA, not applicable.