TABLE 2.
P. aeruginosa proteins with decreased abundance during anaerobic growth
| Genea | Proteine | Gene name | nb | Ratioc | SD |
|---|---|---|---|---|---|
| PA0085 | Conserved hypothetical protein | 3 | 2.15 | 0.26 | |
| PA0100 | Hypothetical protein | 1 | 1.53 | NAd | |
| PA0128 | Conserved hypothetical protein | 9 | 2.10 | 0.35 | |
| PA0139 | Alkyl hydroperoxide reductase subunit C | ahpC | 655 | 2.50 | 1.29 |
| PA0195 | Still frameshift pyridine nucleotide transhydrogenase | pntA | 10 | 2.21 | 0.55 |
| PA0399 | Cystathionine beta-synthase | 6 | 3.39 | 0.52 | |
| PA0447* | Glutaryl-CoA dehydrogenase | gcdH | 24 | 5.30 | 1.04 |
| PA0534 | Conserved hypothetical protein | 4 | 5.46 | 1.51 | |
| PA0588 | Conserved hypothetical protein | 78 | 5.56 | 2.52 | |
| PA0746 | Probable acyl-CoA dehydrogenase | 2 | 2.52 | 0.51 | |
| PA0853 | Probable oxidoreductase | 16 | 2.19 | 0.30 | |
| PA0854 | Fumarate hydratase | fumC2 | 9 | 2.36 | 0.34 |
| PA0870 | Aromatic amino acid aminotransferase | phhC | 24 | 1.74 | 0.22 |
| PA0871 | Pterin-4-alpha-carbinolamine dehydratase | phhB | 27 | 2.37 | 0.57 |
| PA0872 | Phenylalanine-4-hydroxylase | phhA | 60 | 2.11 | 0.65 |
| PA0916 | Conserved hypothetical protein | 6 | 1.93 | 0.28 | |
| PA0997* | Quinolone signal biosynthesis protein | pqsB | 3 | 15.54 | 6.73 |
| PA0998* | Quinolone signal biosynthesis protein | pqsC | 5 | 9.11 | 3.39 |
| PA0999* | 3-Oxoacyl-[acyl-carrier-protein] synthase III | pqsD | 12 | 5.62 | 1.50 |
| PA1002* | Anthranilate synthase component II | phnB | 1 | 2.30 | NA |
| PA1228 | Hypothetical protein | 13 | 2.55 | 0.52 | |
| PA1529 | DNA ligase | lig | 21 | 2.50 | 0.33 |
| PA1574 | Conserved hypothetical protein | 1 | 2.25 | NA | |
| PA1662 | Probable ClpA/B-type protease | 2 | 2.65 | 0.27 | |
| PA1756 | 3′-Phosphoadenosine-5′-phosphosulfate reductase | cysH | 3 | 2.89 | 0.13 |
| PA1772 | Probable methyltransferase | 4 | 2.34 | 0.43 | |
| PA1894 | Hypothetical protein | 9 | 2.48 | 0.94 | |
| PA1964 | Probable ATP-binding component of ABC transporter | 1 | 1.00 | NA | |
| PA2001 | Acetyl-CoA acetyltransferase | atoB | 149 | 1.74 | 1.11 |
| PA2007 | Maleylacetoacetate isomerase | maiA | 10 | 2.45 | 0.47 |
| PA2008 | Fumarylacetoacetase | fahA | 47 | 11.02 | 4.75 |
| PA2009 | Homogentisate 1,2-dioxygenase | hmgA | 4 | 20.39 | 11.50 |
| PA2012* | Probable acyl-CoA carboxylase alpha chain | 7 | 2.24 | 0.19 | |
| PA2014* | Probable acyl-CoA carboxyltransferase beta chain | 69 | 2.14 | 0.44 | |
| PA2044 | Hypothetical protein | 4 | 3.49 | 0.24 | |
| PA2069 | Probable carbamoyl transferase | 10 | 4.11 | 1.13 | |
| PA2081 | Hypothetical protein | 4 | 2.25 | 0.12 | |
| PA2112* | Conserved hypothetical protein | 28 | 3.94 | 0.90 | |
| PA2116 | Conserved hypothetical protein | 35 | 3.67 | 0.97 | |
| PA2194 | Hydrogen cyanide synthase HcnB | hcnB | 9 | 3.26 | 0.50 |
| PA2195 | Hydrogen cyanide synthase HcnC | hcnC | 3 | 4.11 | 0.15 |
| PA2247 | 2-Oxoisovalerate dehydrogenase (alpha subunit) | bkdA1 | 7 | 3.54 | 1.00 |
| PA2248 | 2-Oxoisovalerate dehydrogenase (beta subunit) | bkdA2 | 59 | 2.80 | 1.07 |
| PA2250 | Lipoamide dehydrogenase Val | lpdV | 18 | 2.79 | 0.59 |
| PA2366* | Conserved hypothetical protein | 1 | 2.70 | NA | |
| PA2552* | Probable acyl-CoA dehydrogenase | 13 | 1.99 | 0.70 | |
| PA2553* | Probable acyl-CoA thiolase | 48 | 2.17 | 0.50 | |
| PA2555* | Probable AMP-binding enzyme | 10 | 2.22 | 0.56 | |
| PA2850 | Organic hydroperoxide resistance protein | ohr | 6 | 2.26 | 0.37 |
| PA2939 | Probable aminopeptidase | 3 | 2.67 | 0.80 | |
| PA2981 | Tetraacyldisaccharide 4′-kinase | lpxK | 1 | 13.49 | NA |
| PA3049 | Ribosome modulation factor | rmf | 15 | 3.84 | 0.92 |
| PA3195 | Glyceraldehyde 3-phosphate dehydrogenase | gapA | 1 | 2.76 | NA |
| PA3327 | Probable nonribosomal peptide synthetase | 1 | 2.16 | NA | |
| PA3328 | Probable FAD-dependent monooxygenase | 6 | 4.58 | 1.28 | |
| PA3329* | Hypothetical protein | 1 | 2.08 | NA | |
| PA3331 | Cytochrome P450 | 17 | 5.10 | 2.00 | |
| PA3347 | Hypothetical protein | 4 | 1.96 | 0.20 | |
| PA3365 | Probable chaperone | 1 | 2.35 | NA | |
| PA3366 | Aliphatic amidase | amiE | 1 | 2.00 | NA |
| PA3481 | Conserved hypothetical protein | 1 | 1.54 | NA | |
| PA3537 | Ornithine carbamoyltransferase, anabolic | argF | 1 | 5.57 | NA |
| PA3569 | 3-Hydroxyisobutyrate dehydrogenase | mmsB | 25 | 3.67 | 0.94 |
| PA3570 | Methylmalonate-semialdehyde dehydrogenase | mmsA | 1 | 3.17 | NA |
| PA3842 | Probable chaperone | 8 | 3.17 | 1.44 | |
| PA3919* | Conserved hypothetical protein | 7 | 2.19 | 0.36 | |
| PA4015 | Conserved hypothetical protein | 11 | 2.11 | 0.67 | |
| PA4129* | Hypothetical protein | 3 | 3.75 | 1.17 | |
| PA4132 | Conserved hypothetical protein | 6 | 2.36 | 1.11 | |
| PA4217 | Flavin-containing monooxygenase | phzS | 5 | 5.09 | 1.26 |
| PA4362 | Hypothetical protein | 2 | 2.17 | 0.29 | |
| PA4412* | MurG protein | murG | 1 | 3.28 | NA |
| PA4498 | Probable metallopeptidase | 4 | 2.00 | 0.06 | |
| PA5100 | Urocanase | hutU | 10 | 4.50 | 1.23 |
| PA5410 | Probable ring hydroxylating dioxygenase, alpha subunit | 1 | 2.76 | NA |
a
Genes marked with an asterisk (*) were identified as down-regulated during anaerobic growth (1).
b
Number of peptides identified and quantified for each protein.
c
Values represent relative protein abundance, or the ratio of protein expression in cells grown aerobically to protein expression in cells grown anaerobically.
d
NA, not applicable.
e
CoA, coenzyme A; FAD, flavin adenine dinucleotide.