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. 2005 Nov 25;1(3):e29. doi: 10.1371/journal.ppat.0010029

Figure 6. PfSUB2PD Inhibits MESH in a Selective, Dose-Dependent Manner.

Figure 6

(A) Shedding of AMA1 and MSP1 from isolated merozoites was assayed in the presence of the indicated additives. Two different batches (1 and 2) of purified PfSUB2PD were tested, in each case alongside a similar dilution of the membrane flow-through (Conc FT) from the final step of concentration by ultrafiltration. Control additives used were bovine serum albumin (BSA), purified PfSUB1PD, and EDTA. No effect on processing was seen if PfSUB2PD was added to buffer control reactions at the end of the incubation period (extreme right-hand lane). The lower panel shows a titration of inhibition of MSP1 shedding in the presence of 2-fold serially decreasing concentrations of PfSUB2PD (from 1.2 μM to 0.075 μM).

(B) Effect of propeptides on PfSUB1 activity. Progress curves showing cleavage of substrate pepF1-6R by recombinant PfSUB1 before and following addition of 600 nM purified PfSUB2PD or PfSUB1PD, or BSA. Note that this concentration is in at least 300-fold molar excess over the amount of recombinant PfSUB1 present in the assay. The time point of addition of test proteins is arrowed.