Table 1.

List of required equipment.

No.	Item	Qty	Description	Supplier
Polymer embedding and specimen extraction
1	Permanent Life Support (PLS)	1	Embedded and investigated ML	Getinge, Rastatt, Germany
2	Technovit® embedding molds with hexagonal inserts	18	Embedding molds for specimen extraction	Morphisto, Offenbach, Germany
3	Micro V G222E O2 monitor	1	Monitoring O2 during CO2 use	GfG, Dortmund, Germany
4	MH 20 magnetic stirrer	1	Stirring of polymer solution	Carl Roth, Karlsruhe, Germany
5	Testo 835-T1 infrared thermometer	1	Monitoring polymerization temperature	Testo, Titisee-Neustadt, Germany
6	Dremel 4000	1	Cutting off connector ports of ML	Dremel, Racine, WI, USA
7	TC-SB 305 U band saw	1	Cutting out specimens from embedded ML	Einhell, Landau, Germany
Microtome sectioning
8	HM355S microtome	1	Microtome sectioning of embedded ML specimens	Thermo Fisher, Waltham, MA, USA
9	R16D Tungsten-carbide D-knife 16 cm	1		Gigatome, Roggenburg, Germany
10	Cylindrical microtome mounting with fixation of 25 mm diameter specimens	1		OTH Regensburg, Regensburg, Germany
11	Superfrost Ultra Plus® glass slides	1 pck	Glass slides	Thermo Fisher, Waltham, MA, USA
Image acquisition and processing
12	DMi8 inverse microscope with a LED 5 light source	1	Imaging of sections from embedded ML	Leica Microsystems, Wetzlar, Germany
13	K3C RGB camera	1	Transmitting light microscopy
14	K5 grayscale camera	1	Fluorescence microscopy
15	DFT51011 filter cube (420–450 nm, 506–532 nm, 578–610 nm, 666–724 nm)	1	Optical filter for fluorescence microscopy
16	DFT5 filter wheel (420–460 nm, 500–540 nm, 565–615 nm, 662–738 nm)	1
17	LAS X 3.10 software	1	Control software for DMi8 microscope
18	Adobe Photoshop CS5 64 bit	1	Imaging software to reduce background	Adobe, San José, CA, USA