Table 2.
List of required reagents and solutions.
| No. | Reagent | Art. No./supplier |
|---|---|---|
| Embedding polymers | ||
| 19 | HistoCURE 8100 Set 500 mL (2 sets) | 12226.K0500, Morphisto, Offenbach, Germany |
| 20 | Technovit® 3040 set | 12226.K0180, Morphisto, Offenbach, Germany |
| Fixative and buffer solutions | ||
| 21 | NaCl 0.9% | 1310181, Fresenius-Kabi, Bad Homburg, Germany |
| 22 | Paraformaldehyde | 104005, Merck, Darmstadt, Germany |
| 23 | Methanol | 4627, Carl Roth, Karlsruhe, Germany |
| 24 | NaOH flakes | LC4994.1, LaboChem, Ruse, Bulgaria |
| 25 | Na2HPO4 | 106579, Merck, Darmstadt, Germany |
| 26 | KCl | HN02.2, Carl Roth, Karlsruhe, Germany |
| 27 | KH2PO4 | 104873, Merck, Darmstadt, Germany |
| 28 | NaCl | 106400, Merck, Darmstadt, Germany |
| 29 | Tris-Base | T1503–1 kg, Sigma-Aldrich, Steinheim, Germany |
| 30 | Tris-HCl | 9090.2, Carl Roth, Karlsruhe, Germany |
| 31 | Bovine serum albumin (BSA), Frakt. V | 8076.2, Carl Roth, Karlsruhe, Germany |
| 32 | Gelantin from cold water fish skin | G-7765, Sigma-Aldrich, Steinheim, Germany |
| 33 | Normal goat serum (NGS) | G-9023, Sigma-Aldrich, Steinheim, Germany |
| 34 | Normal donkey serum (NDS) | D-9663, Sigma-Aldrich, Steinheim, Germany |
| Histological staining solutions | ||
| 35 | Mayeŕs hemalum solution | 1.09249.0500, Sigma-Aldrich, Steinheim, Germany |
| 36 | Eosin | 15935, Merck, Darmstadt, Germany |
| 37 | May-Grunwald stain | 63595, Sigma-Aldrich, Steinheim, Germany |
| 38 | Giemsa stain | 48900, Sigma-Aldrich, Steinheim, Germany |
| Antibodies | ||
| 39 | Rabbit, anti human, anti-Myeloperoxidase (MPO) | A0398, DAKO Cytomation, Wiesentheid, Germany |
| 40 | Rabbit, anti human, anti-von Willebrand factor (vWF) | A0082, DAKO Cytomation, Wiesentheid, Germany |
| 41 | Rabbit, anti human, isotype control antibody | 31235, Invitrogen, Waltham, MA, USA |
| 42 | Goat, anti rabbit, biotinylated antibody | BA-1000, Vector Laboratories, Newark, CA, USA |
| 43 | Donkey, anti rabbit AlexaFluor 647 | A-31573, Invitrogen, Waltham, MA, USA |
| Agents and stains for immunhistochemistry (IHC) and for immunohistofluorescence (IHF) | ||
| 44 | Vectastain® Elite® ABC Kit, Peroxidase (Standard) | PK-6100, Vector Laboratories, Newark, CA, USA |
| 45 | Histogreen | E109, Linaris/Biozol Diagnostica, Eching, Germany |
| 46 | DAB-Liquid | K3468, Dako/Agilent, Santa Clara, CA, USA |
| 47 | SYTOX™ Green | S-7020, Thermo Fisher, Waltham, MA, USA |
| Mounting agents | ||
| 48 | EntellanTM | 1.07961.0500, Merck, Darmstadt, Germany |
| 49 | VectaMount® AQ | H-5501, Vector Laboratories, Newark, CA, USA |
| 50 | Flouromount-G™ | 00–4958-02, Thermo Fisher, Waltham, MA, USA |
| 51 | Flouromount-G™ DAPI | 00-4959-52, Thermo Fisher, Waltham, MA, USA |
| Miscellaneous reagents | ||
| 52 | Ethanol absolute | 107017, Merck, Darmstadt, Germany |
| 53 | HCl | 1.00317.0510, Merck, Darmstadt, Germany |
| 54 | Acetic acid 100% | 1.00063.1011, Merck, Darmstadt, Germany |
| 55 | Xylene, (mixture of isomers) ≥98% | 28973.363, VWR, Radnor, PA, USA |
| 56 | H2O2 | 386790, Merck, Darmstadt, Germany |
| 57 | Trypsin-EDTA | T4174, Sigma-Aldrich, Steinheim, Germany |
| 58 | CaCl2 (1M) | 21114, Honeywell Fluka, Sleeze, Germany |
| 59 | Citric buffer (pH 6) | ZUC 028-500, Zytomed, Berlin, Germany |
| 60 | Glycerol >99% | SHBL3980, Sigma-Aldrich, Steinheim, Germany |