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. 2005 Nov 25;1(3):e34. doi: 10.1371/journal.ppat.0010034

Figure 1. TLR2 and TLR4 Are Partially Responsible for Induction of Cytokine Production by M. tuberculosis .

Figure 1

(A) CHO cells cotransfected with CD14 and TLR2 (CD14/TLR2, hatched bars) induced potent expression of CD25 on the cell membrane as measured by FACS analysis, after stimulation with both a sonicated M. tuberculosis (MTB-son, 1 μg/ml) or whole mycobacteria (MTB-whole, 1 × 107/ml). In contrast, cells transfected with CD14 and TLR4 (CD14/TLR4, black bars) were only moderately activated by whole M. tuberculosis, but not by the sonicated material. LPS (1 μg/ml) and Pam3Cys (5 μg/ml) served as control stimuli for TLR4 and TLR2, respectively. As controls, cells transfected only with CD14 were used (white bars).

(B and C) Stimulation of TNF production by M. tuberculosis in peritoneal macrophages of mice deficient for TLR2 (TLR2-KO) (B) or TLR4 (TLR4-def) (C). Groups of five mice were stimulated with the indicated reagents, and the experiment was repeated twice. Medium-stimulated cells resulted in cytokine concentrations below detection limit.

Data are presented as mean ± SD (n = 5; *p < 0.05).