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. 2005 Nov 25;1(3):e34. doi: 10.1371/journal.ppat.0010034

Figure 6. NOD2 and TLR2 Have Synergistic Effects on Cytokine Production.

Figure 6

(A) MNCs isolated from five healthy volunteers with wild-type NOD2 were costimulated with 1 μg/ml MDP and increasing concentrations of 19-kDa lipoprotein (indicated on the x-axis). TNF was measured after 24 h of stimulation at 37 °C by specific RIA.

(B) The synergistic effects observed in the five control volunteers, as well as in five Crohn's disease patients heterozygous for NOD2 mutations (NOD2-het), and in five patients with the wild-type NOD2 allele (NOD2-WT), was lost in patients homozygous for the NOD2 3020insC mutation. Medium-stimulated cells resulted in cytokine concentrations below detection limit. Data are presented as mean ± SD (*p < 0.05).