Figure 7.

Endostatin does not induce cleavage of caspase-3 into its active forms. HDMEC were seeded on either plastic or collagen I coated tissue culture dishes, and following removal of non-adherent cells by washing, were stimulated with either 50 ng/ml VEGF alone or in combination with 100, 500 or 2500 ng/ml recombinant endostatin for 48 h. Cells were collected by trypsinization or collagenase treatment and total protein lysates were generated. Western blots for active caspase-3 were performed as described in the methods section.