Table 2.
Anabolic demand of Saccharomyces cerevisiae during aerobic glucose-limited chemostat culture at different dilution rates. The data are given in μmol (g cell dry mass)-1 and are calculated from the cellular composition.
| Compound | D = μ = 0.15 h-1 | D = μ = 0.30 h-1 | D = μ = 0.40 h-1 |
| Glucose 6-phosphate | 2089 | 1694 | 1431 |
| Erythrose 4-phosphate | 243 | 281 | 307 |
| Ribose 5-phosphate | 115 | 127 | 141 |
| Glyceraldehyde 3-phosphate | 77 | 77 | 77 |
| Phosphoglycerate (for lipids, nucleotides) | 44 | 44 | 44 |
| Phosphoglycerate (for serine + cysteine) | 343 | 397 | 433 |
| Phosphoglycerate/Oxaloacetate (for glycine)a | 236 | 273 | 298 |
| Oxaloacetate (for threonine, methionine, isoleucine) | 393 | 455 | 496 |
| Oxaloacetate (for others) | 290 | 332 | 364 |
| Phosphoenolpyruvate | 457 | 529 | 577 |
| Pyruvate (for alanine)b | 304 | 352 | 384 |
| Pyruvate (mitochondrial, for others) | 981 | 1135 | 1237 |
| Acetyl-CoA (cytosolic) | 2108 | 2142 | 2164 |
| Acetyl-CoA (mitochondrial) | 216 | 250 | 273 |
| 2-Oxoglutarate | 1008 | 1166 | 1272 |
| NADPH | 10088 | 11206 | 11956 |
a The actual precursor demand for glycine biosynthesis depends on the contribution of the serine pathway (from phosphoglycerate) and threonine aldolase (from oxaloacetate). Based on the relative activity of both pathways, the corresponding demand has to be added appropriately to phosphoglycerate and oxaloacetate, respectively.
b The demand of cytosolic and mitochondrial pyruvate for alanine synthesis depends on the relative contribution of cytosolic and mitochondrial route to alanine synthesis.