Fig. 2. Anisotropic mechanical properties of Arabidopsis cell walls in the cortex layer.

(A) Orientations of cell walls and confocal images of the cortex plane visualized with p35S::LTI6b-GFP. Regions where imaging was performed are indicated for the meristem (blue), transition (pink), and elongation zone (green). (B) Confocal images of the meristem (left), transition zone (middle), and elongation (right) zones, used to locate the cortex planes to generate the following heatmaps. (C) Heatmaps of the frequency shift in cells in the meristem (50 by 40 μm, left), transition (32 by 32 μm, middle), and elongation zone (30 by 60 μm, right). (D) Quantification of the frequency shifts at cell walls. (E) Heatmaps of the linewidth in cells at the meristem (left), transition zone (middle) and elongation zone (right). (F) Quantification linewidth at cell walls. n = 8 to 10 different roots for each zone. A paired t test was used to obtain the statistics for individual zones. P values are reported as ns > 0.05, **P < 0.01, ***P < 0.001.