Fig. 3. Mechanical properties of cell walls and cytoplasm in the elongation zone.

(A) Confocal image of the median plane of a root elongation zone expressing p35S::LTI6b-GFP. The yellow rectangle indicates the area scanned with the Brillouin microscope. (B) Heatmaps (10 by 100 μm) of frequency shift (top) and linewidth (bottom). (C) Focused heatmaps (10 by 42 μm) of epidermis and cortex cells showing frequency shift (upper) and linewidth (lower). (D) Quantification of the frequency shift (left) and linewidth (right) of cell walls. (E) Quantification of the frequency shift (left) and linewidth (right) of the cytoplasm. n = 10 roots for each tissue layer. A repeated-measures ANOVA with Holm correction was used for statistical analysis. epi, epidermis; cx, cortex; endo, endodermis; per, pericycle.