5.

Variation of absorbance at 425 nm of tartrazine dye at pH 2 (A) and pH 7.0 (B) over time under different temperatures. Experimental conditions: 0.1 g of inactivated BSY was added to 50 mL of buffer solution containing tartrazine dye at a concentration of 10 mg·L^–1. Samples were incubated at 10, 25, 37, and 90 °C.