Skip to main content
. 2026 Feb 5;11(6):10505–10517. doi: 10.1021/acsomega.5c11877

2. PTM Levels in Forced Degradation Samples.

Average ± SD M254 Oxidation, % M360 Oxidation, % N386 Deamidation, % N386 NH3 loss, % N392 NH3 loss, % M399 Oxidation, % N152 NH3 loss, % N158 Deamidation, %
mAb Non stressed 4.64 ± 14.40 0.60 ± 1.94 0.72 ± 0.40 0.32 ± 0.05 0.75 ± 0.09 0.51 ± 0.04 0.00 ± 0.00 1.87 ± 3.22
Heat stressed 7.47 ± 0.57 1.00 ± 0.03 1.74 ± 0.72 0.42 ± 0.02 0.81 ± 0.03 3.85 ± 0.29 0.02 ± 0.00 3.21 ± 0.17
Photo stressed 27.00 ± 8.52 4.11 ± 1.00 0.86 ± 0.45 0.35 ± 0.04 0.82 ± 0.07 0.57 ± 0.03 0.00 ± 0.00 7.43 ± 0.46
UPBH Non stressed 3.75 ± 0.13 0.66 ± 0.06 1.23 ± 0.33 0.98 ± 0.07 0.34 ± 0.04 1.81 ± 0.27 0.05 ± 0.01 0.26 ± 0.04
Heat stress 44.58 ± 0.52 11.41 ± 0.68 24.83 ± 17.75 0.83 ± 0.30 0.65 ± 0.27 22.80 ± 0.80 0.05 ± 0.01 2.32 ± 0.30
Photo stressed 95.88 ± 0.33 58.93 ± 0.43 7.60 ± 9.98 0.99 ± 0.17 0.50 ± 0.17 58.79 ± 1.04 0.06 ± 0.00 0.36 ± 0.11
UPBH (Buffer exchanged) Non stressed 5.84 ± 0.14 0.87 ± 0.04 2.32 ± 2.13 0.88 ± 0.06 0.29 ± 0.03 2.48 ± 0.79 0.03 ± 0.01 0.25 ± 0.05
Heat stress 40.76 ± 1.92 7.55 ± 0.70 18.42 ± 23.84 2.39 ± 1.42 0.32 ± 0.14 21.39 ± 24.16 0.04 ± 0.01 0.37 ± 0.04
Photo stressed 97.12 ± 0.61 70.69 ± 0.45 1.20 ± 0.83 0.89 ± 0.15 0.43 ± 0.09 77.22 ± 0.47 0.06 ± 0.03 0.32 ± 0.11
Average ± SD N158 NH3 loss, % K328 Glycation, % N327 NH3 loss, % N317 Deamidation, % N317 NH3 loss, % M430 Oxidation, % N436 Deamidation, % Q421 Deamidation, %
mAb Non stressed 0.04 ± 0.01 0.04 ± 0.01 0.33 ± 0.25 0.64 ± 0.27 0.05 ± 0.07 0.93 ± 9.64 0.29 ± 0.12 0.12 ± 0.04
Heat stressed 0.05 ± 0.01 0.04 ± 0.00 0.46 ± 0.07 1.18 ± 0.21 0.61 ± 0.19 2.03 ± 0.10 0.31 ± 0.10 0.39 ± 0.06
Photo stressed 0.05 ± 0.01 0.04 ± 0.01 0.43 ± 0.26 0.71 ± 0.22 0.16 ± 0.13 15.61 ± 8.48 0.24 ± 0.07 0.09 ± 0.04
UPBH Non stressed 0.03 ± 0.01 0.76 ± 0.17 0.04 ± 0.01 0.69 ± 0.22 1.87 ± 0.25 0.66 ± 0.02 0.01 ± 0.01 0.02 ± 0.02
Heat stress 0.03 ± 0.00 2.24 ± 0.04 0.04 ± 0.00 1.87 ± 0.52 2.05 ± 0.45 10.71 ± 0.22 0.34 ± 0.29 0.11 ± 0.11
Photo stressed 0.03 ± 0.01 0.89 ± 0.04 0.08 ± 0.00 0.95 ± 0.28 1.76 ± 0.33 68.56 ± 10.36 0.07 ± 0.05 0.02 ± 0.02
UPBH (Buffer exchanged) Non stressed 0.04 ± 0.01 0.49 ± 0.12 0.03 ± 0.01 0.23 ± 0.21 1.57 ± 0.22 1.60 ± 0.04 0.09 ± 0.08 0.03 ± 0.03
Heat stress 0.04 ± 0.01 0.38 ± 0.04 3.49 ± 2.13 2.20 ± 0.50 4.88 ± 0.87 19.95 ± 15.93 0.48 ± 0.54 0.11 ± 0.06
Photo stressed 0.05 ± 0.02 0.57 ± 0.11 0.11 ± 0.01 0.49 ± 0.06 1.60 ± 0.33 97.32 ± 0.50 0.00 ± 0.00 0.00 ± 0.00