3. Detected Glycan Species .
| Average ± SD | G0F | G0F-Gn | G1F | M5 | Aglycosylated | G0-Gn | |
|---|---|---|---|---|---|---|---|
| mAb | Non stressed | 77.44 ± 1.19 | 4.56 ± 0.11 | 4.97 ± 0.09 | 0.55 ± 0.03 | 4.69 ± 0.96 | 0.97 ± 0.12 |
| Heat stress | 79.66 ± 1.21 | 3.92 ± 0.24 | 5.04 ± 0.06 | 0.43 ± 0.05 | 3.62 ± 0.61 | 0.81 ± 0.13 | |
| Photo stressed | 76.91 ± 2.92 | 4.15 ± 0.34 | 5.24 ± 0.49 | 0.56 ± 0.06 | 5.82 ± 2.71 | 0.88 ± 0.20 | |
| UPBH | Non stressed | 73.71 ± 0.98 | 4.10 ± 0.13 | 5.34 ± 0.19 | 0.40 ± 0.25 | 8.63 ± 2.00 | 0.61 ± 0.21 |
| Heat stress | 76.70 ± 1.56 | 4.09 ± 0.31 | 5.44 ± 0.13 | 0.50 ± 0.06 | 6.44 ± 1.53 | 0.55 ± 0.11 | |
| Photo stressed | 74.79 ± 2.04 | 4.42 ± 0.17 | 5.68 ± 0.21 | 0.53 ± 0.15 | 7.41 ± 1.96 | 0.64 ± 0.11 | |
| UPBH (Buffer exchanged) | Non stressed | 75.62 ± 0.40 | 4.18 ± 0.40 | 5.45 ± 0.48 | 0.36 ± 0.12 | 8.00 ± 2.47 | 0.34 ± 0.09 |
| Heat stress | 84.29 ± 2.70 | 2.44 ± 0.82 | 0.85 ± 0.60 | nd | 12.03 ± 2.49 | nd | |
| Photo stressed | 80.08 ± 0.66 | 4.49 ± 0.04 | 6.26 ± 0.21 | 0.05 ± 0.03 | 2.08 ± 0.66 | 0.08 ± 0.05 | |
| Average ± SD | G1F-Gn | G0 | G2F | M7 | M8 | M6 | |
|---|---|---|---|---|---|---|---|
| mAb | Non stressed | 1.31 ± 0.07 | 4.48 ± 0.10 | 0.25 ± 0.04 | 0.21 ± 0.21 | 0.42 ± 0.05 | 0.14 ± 0.03 |
| Heat stress | 1.20 ± 0.05 | 4.37 ± 0.10 | 0.27 ± 0.02 | 0.18 ± 0.18 | 0.36 ± 0.02 | 0.14 ± 0.01 | |
| Photo stressed | 1.08 ± 0.35 | 4.53 ± 0.54 | 0.32 ± 0.11 | 0.14 ± 0.14 | 0.28 ± 0.23 | 0.10 ± 0.09 | |
| UPBH | Non stressed | 1.12 ± 0.05 | 4.64 ± 0.13 | 0.34 ± 0.13 | 0.26 ± 0.26 | 0.77 ± 0.23 | 0.07 ± 0.06 |
| Heat stress | 0.99 ± 0.24 | 4.79 ± 0.35 | 0.39 ± 0.17 | 0.01 ± 0.01 | 0.03 ± 0.02 | 0.07 ± 0.03 | |
| Photo stressed | 0.96 ± 0.18 | 4.73 ± 0.56 | 0.50 ± 0.10 | 0.14 ± 0.14 | 0.09 ± 0.07 | 0.09 ± 0.09 | |
| UPBH (Buffer exchanged) | Non stressed | 0.63 ± 0.35 | 4.31 ± 0.29 | 0.33 ± 0.18 | 0.12 ± 0.12 | 0.65 ± 0.30 | 0.00 ± 0.01 |
| Heat stress | nd | 0.30 ± na | 0.08 ± 0.14 | nd | nd | nd | |
| Photo stressed | 1.20 ± 0.26 | 5.02 ± 0.38 | 0.55 ± 0.05 | 0.03 ± 0.03 | 0.14 ± 0.10 | 0.01 ± 0.01 | |
nd: not detected, na: not applicable (not detected in all three injections).