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. 2026 Feb 5;11(6):10505–10517. doi: 10.1021/acsomega.5c11877

3. Detected Glycan Species .

Average ± SD G0F G0F-Gn G1F M5 Aglycosylated G0-Gn
mAb Non stressed 77.44 ± 1.19 4.56 ± 0.11 4.97 ± 0.09 0.55 ± 0.03 4.69 ± 0.96 0.97 ± 0.12
Heat stress 79.66 ± 1.21 3.92 ± 0.24 5.04 ± 0.06 0.43 ± 0.05 3.62 ± 0.61 0.81 ± 0.13
Photo stressed 76.91 ± 2.92 4.15 ± 0.34 5.24 ± 0.49 0.56 ± 0.06 5.82 ± 2.71 0.88 ± 0.20
UPBH Non stressed 73.71 ± 0.98 4.10 ± 0.13 5.34 ± 0.19 0.40 ± 0.25 8.63 ± 2.00 0.61 ± 0.21
Heat stress 76.70 ± 1.56 4.09 ± 0.31 5.44 ± 0.13 0.50 ± 0.06 6.44 ± 1.53 0.55 ± 0.11
Photo stressed 74.79 ± 2.04 4.42 ± 0.17 5.68 ± 0.21 0.53 ± 0.15 7.41 ± 1.96 0.64 ± 0.11
UPBH (Buffer exchanged) Non stressed 75.62 ± 0.40 4.18 ± 0.40 5.45 ± 0.48 0.36 ± 0.12 8.00 ± 2.47 0.34 ± 0.09
Heat stress 84.29 ± 2.70 2.44 ± 0.82 0.85 ± 0.60 nd 12.03 ± 2.49 nd
Photo stressed 80.08 ± 0.66 4.49 ± 0.04 6.26 ± 0.21 0.05 ± 0.03 2.08 ± 0.66 0.08 ± 0.05
Average ± SD G1F-Gn G0 G2F M7 M8 M6
mAb Non stressed 1.31 ± 0.07 4.48 ± 0.10 0.25 ± 0.04 0.21 ± 0.21 0.42 ± 0.05 0.14 ± 0.03
Heat stress 1.20 ± 0.05 4.37 ± 0.10 0.27 ± 0.02 0.18 ± 0.18 0.36 ± 0.02 0.14 ± 0.01
Photo stressed 1.08 ± 0.35 4.53 ± 0.54 0.32 ± 0.11 0.14 ± 0.14 0.28 ± 0.23 0.10 ± 0.09
UPBH Non stressed 1.12 ± 0.05 4.64 ± 0.13 0.34 ± 0.13 0.26 ± 0.26 0.77 ± 0.23 0.07 ± 0.06
Heat stress 0.99 ± 0.24 4.79 ± 0.35 0.39 ± 0.17 0.01 ± 0.01 0.03 ± 0.02 0.07 ± 0.03
Photo stressed 0.96 ± 0.18 4.73 ± 0.56 0.50 ± 0.10 0.14 ± 0.14 0.09 ± 0.07 0.09 ± 0.09
UPBH (Buffer exchanged) Non stressed 0.63 ± 0.35 4.31 ± 0.29 0.33 ± 0.18 0.12 ± 0.12 0.65 ± 0.30 0.00 ± 0.01
Heat stress nd 0.30 ± na 0.08 ± 0.14 nd nd nd
Photo stressed 1.20 ± 0.26 5.02 ± 0.38 0.55 ± 0.05 0.03 ± 0.03 0.14 ± 0.10 0.01 ± 0.01
a

nd: not detected, na: not applicable (not detected in all three injections).