Figure 2: ATP8B1 is enriched toward the base of stereocilia.
(A) ATP8B1-HA immunofluorescence showing OHC specific expression. (B,C) ATP8B1-HA IF is enriched towards the stereocilia base and the apical hair cell membrane. (D) Average normalized fluorescence intensity profiles of actin and ATP8B1 along short (row 2) and long (row 1) stereocilia. Position is normalized from 0 (base) to 1 (tip), and intensities are normalized to total signal per stereocilium. Shaded regions indicate SEM. (Scale bar: 5 μm and 0.5 μm in stereocilia inset)
