5.

Condition-dependence of NanoBondy reaction. (A) Buffer- and temperature-dependence of NanoBondy reaction. 2H5 R72C anti-CD45 NanoBondy was incubated with CD45d, each at 10.5 μM, in the indicated buffer and temperature before SDS-PAGE with Coomassie staining. Ctrl refers to the lane containing anti-IgG NanoBondy with CD45d for 120 min. Representative gel from two independent experiments. (B) pH-dependence of NanoBondy reaction. The reaction was evaluated as in (A) with HBS-MES buffer at the indicated pH at 37 °C. Ctrl refers to the lane containing anti-IgG NanoBondy with CD45d for 120 min. The experiment was conducted once.