Figure 2.

Hst5 decreases corneal opacification in corneal alkali injury. (A) White light images were taken using slit-lamp biomicroscopy and images showed the area of corneal epithelial injury of nontreated and the Hst5 (20 µM). Corneal opacity was significantly lower in 20(S)-OHC-treated mice than in the BSS group. (B) The histogram represents the scoring of corneal opacity at the indicated time after alkali injury with BSS or Hst5 treatment. The values are expressed as floating bars (min to max) with mean (—) and are representative of five animals per group. Un = Uninjured, Baseline. Corneal opacification was scored using a system of scaling from 0 to 4 described in materials and methods. All values are expressed as mean ± SEM (n = 5 per group). Statistical significance was determined by two-way ANOVA with multiple comparisons. **P < 0.01, ***P < 0.001, ****P < 0.0001.