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. 2026 Feb 17;15(2):20. doi: 10.1167/tvst.15.2.20

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Figure 4. — Hst5 inhibits the infiltration of F4/80 positive macrophages induced by alkali burn injured murine corneas. (A) The uninjured cornea (Un). Immunofluorescent staining of F4/80 antibody in cross-sectioned murine cornea demonstrated that F4/80-positive macrophages infiltrated the corneal stroma on day 14 after the alkali burn induction. Fluorescence images showing infiltration of F4/80 positive cells (white arrows) in the alkali burn injured group at day 14 (B). Hst5 inhibits F4/80 positive inflammatory cells recruitment (C). (D) A bar graph representing the mean ± SD of F4/80⁺ cells number counted from three eyes per treatment group. Corneal sections were stained with immunofluorescent antibodies for positive cells, as shown (Scale bar: 100 µm).