Fig. 6.
Inverse metabolic engineering and theoretical assessment of the principles underlying its mechanism. a. Transcriptional changes of the YALI0B21846g, YALI0E20691g, and YALI0A09768g genes with high |log2FC| values at 24 h. b. Succinic acid titer and yield of the control strain, recombinant strains obtained through inverse metabolic engineering, and evolved strains at 120 h. c. Growth performance of the control strain, recombinant strains obtained through inverse metabolic engineering, and evolved strains. d. Metabolic map of the tyrosine degradation pathway. 4-Hppd: 4-hydroxyphenylpyruvate dioxygenase; Hmga: homogentisate 1,2-dioxygenase; Faha: fumarylacetoacetase. e. Metabolic diagram of succinic acid production from glucose via glycolysis or the hexose monophosphate pathway. EMP: Embden-Meyerhof-Parnas pathway; TDP: Tyrosine degradation pathway; HMP: Hexose monophosphate pathway. f. Lipid content of strains ST-110 and ST-113 relative to dry cell weight. g. The TDP activated in the process of adaptive evolution is capable of enhancing carbon flux within the HMP pathway. ALE: Adaptive laboratory evolution.