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. 2001 Nov;127(3):749–764.

Table I.

Comparison of metabolic levels in wild-type developing potato tubers with those in tubers of transgenic potato plants

WT cPGM29 cPGM44 cPGM66 WT pPGM5 pPGM9 pPGM60 WT AGP-85 AGP-93
Ala 1.00 ± 0.07 0.57 ± 0.11 0.51 ± 0.11 1.48 ± 0.06 1.00 ± 0.13 0.26 ± 0.04 0.40 ± 0.07 0.22 ± 0.05 1.00 ± 0.19 0.32 ± 0.04 0.16 ± 0.03
Arg 1.00 ± 0.08 0.87 ± 0.18 0.74 ± 0.17 2.21 ± 0.14 1.00 ± 0.07 0.60 ± 0.05 0.88 ± 0.24 0.58 ± 0.13 1.00 ± 0.18 0.74 ± 0.05 0.47 ± 0.08
Asn 1.00 ± 0.18 0.87 ± 0.15 0.84 ± 0.17 0.65 ± 0.03 1.00 ± 0.15 0.57 ± 0.16 0.65 ± 0.19 0.27 ± 0.08 1.00 ± 0.24 0.61 ± 0.09 0.40 ± 0.12
Asp 1.00 ± 0.04 0.92 ± 0.06 0.88 ± 0.05 1.15 ± 0.05 1.00 ± 0.12 0.62 ± 0.09 0.53 ± 0.06 0.55 ± 0.10 1.00 ± 0.09 0.57 ± 0.06 0.41 ± 0.04
β-Ala 1.00 ± 0.13 0.66 ± 0.10 0.93 ± 0.15 0.99 ± 0.11 1.00 ± 0.16 0.42 ± 0.06 0.40 ± 0.05 0.29 ± 0.05 1.00 ± 0.18 0.58 ± 0.07 0.55 ± 0.15
Cys n.d. n.d. n.d. n.d. 1.00 ± 0.05 0.75 ± 0.08 n.d. n.d. n.d. n.d. n.d.
GABA 1.00 ± 0.04 0.84 ± 0.07 0.74 ± 0.09 0.41 ± 0.03 1.00 ± 0.13 0.65 ± 0.09 0.63 ± 0.06 0.83 ± 0.08 1.00 ± 0.13 0.82 ± 0.10 0.79 ± 0.18
Glu 1.00 ± 0.03 1.04 ± 0.06 0.95 ± 0.05 1.12 ± 0.04 1.00 ± 0.14 0.29 ± 0.11 0.54 ± 0.04 0.61 ± 0.12 1.00 ± 0.08 1.06 ± 0.11 0.75 ± 0.10
Gln 1.00 ± 0.22 1.02 ± 0.23 0.98 ± 0.20 1.20 ± 0.21 1.00 ± 0.11 0.54 ± 0.05 1.00 ± 0.38 0.51 ± 0.11 1.00 ± 0.09 0.84 ± 0.07 0.63 ± 0.19
Gly 1.00 ± 0.11 0.83 ± 0.13 0.64 ± 0.06 0.97 ± 0.08 1.00 ± 0.08 0.94 ± 0.12 0.63 ± 0.08 0.52 ± 0.09 1.00 ± 0.06 0.98 ± 0.09 0.85 ± 0.17
His n.d. n.d. n.d. n.d. n.d. n.d. n.d. n.d. n.d. n.d. n.d.
Homo-Ser 1.00 ± 0.09 1.24 ± 0.24 1.27 ± 0.05 1.51 ± 0.17 1.00 ± 0.17 0.55 ± 0.04 1.11 ± 0.17 1.07 ± 0.17 1.00 ± 0.06 1.22 ± 0.09 1.11 ± 0.10
Ile 1.00 ± 0.08 0.81 ± 0.09 0.68 ± 0.12 1.66 ± 0.11 1.00 ± 0.31 0.30 ± 0.03 1.06 ± 0.26 0.65 ± 0.12 1.00 ± 0.14 1.02 ± 0.09 0.62 ± 0.08
Leu 1.00 ± 0.19 0.68 ± 0.15 0.65 ± 0.21 3.07 ± 0.50 1.00 ± 0.13 0.39 ± 0.06 1.17 ± 0.37 0.49 ± 0.13 1.00 ± 0.21 0.98 ± 0.15 0.49 ± 0.06
Lys 1.00 ± 0.08 0.60 ± 0.07 0.49 ± 0.14 2.53 ± 0.19 1.00 ± 0.11 0.67 ± 0.03 1.04 ± 0.44 0.41 ± 0.12 1.00 ± 0.22 0.52 ± 0.04 0.26 ± 0.03
Met 1.00 ± 0.04 0.76 ± 0.07 0.49 ± 0.12 1.11 ± 0.05 1.00 ± 0.13 0.56 ± 0.05 0.92 ± 0.18 0.67 ± 0.14 1.00 ± 0.14 0.72 ± 0.08 0.49 ± 0.10
Orn 1.00 ± 0.17 0.76 ± 0.14 0.87 ± 0.23 1.67 ± 0.20 1.00 ± 0.11 0.45 ± 0.10 1.10 ± 0.30 0.63 ± 0.12 1.00 ± 0.18 1.01 ± 0.15 0.77 ± 0.14
5-Oxy-Pro 1.00 ± 0.14 0.74 ± 0.15 0.97 ± 0.05 0.99 ± 0.13 1.00 ± 0.13 0.35 ± 0.04 1.07 ± 0.37 0.46 ± 0.14 1.00 ± 0.11 0.82 ± 0.08 0.64 ± 0.17
Phe 1.00 ± 0.06 0.51 ± 0.08 0.36 ± 0.11 1.23 ± 0.05 1.00 ± 0.16 0.67 ± 0.23 0.83 ± 0.23 0.34 ± 0.08 1.00 ± 0.14 0.46 ± 0.09 0.18 ± 0.02
Pro 1.00 ± 0.09 1.33 ± 0.29 0.51 ± 0.12 2.41 ± 0.52 1.00 ± 0.12 0.53 ± 0.02 0.45 ± 0.09 0.38 ± 0.11 1.00 ± 0.05 1.24 ± 0.11 0.79 ± 0.14
Ser 1.00 ± 0.10 0.83 ± 0.15 0.57 ± 0.07 1.44 ± 0.08 1.00 ± 0.26 0.52 ± 0.02 0.62 ± 0.09 0.49 ± 0.10 1.00 ± 0.14 0.77 ± 0.08 0.55 ± 0.11
Thr 1.00 ± 0.08 0.82 ± 0.13 0.64 ± 0.08 1.22 ± 0.07 1.00 ± 0.11 0.18 ± 0.02 0.68 ± 0.12 0.56 ± 0.11 1.00 ± 0.14 1.04 ± 0.11 0.76 ± 0.15
Trp 1.00 ± 0.17 0.48 ± 0.14 0.22 ± 0.09 1.46 ± 0.27 1.00 ± 0.12 0.27 ± 0.03 1.40 ± 0.64 0.19 ± 0.06 1.00 ± 0.33 0.37 ± 0.08 0.14 ± 0.02
Tyr 1.00 ± 0.08 0.48 ± 0.11 0.28 ± 0.12 1.37 ± 0.07 1.00 ± 0.12 0.70 ± 0.05 1.23 ± 0.53 0.30 ± 0.10 1.00 ± 0.30 0.43 ± 0.07 0.08 ± 0.01
Val 1.00 ± 0.04 0.93 ± 0.05 0.96 ± 0.05 1.19 ± 0.02 1.00 ± 0.11 0.56 ± 0.09 1.00 ± 0.19 0.86 ± 0.16 1.00 ± 0.06 1.22 ± 0.07 0.92 ± 0.12
α-Ketogluterate 1.00 ± 0.07 1.89 ± 0.23 1.79 ± 0.36 0.75 ± 0.12 n.d. n.d. n.d. n.d. n.d. n.d. n.d.
Ascorbate 1.00 ± 0.25 0.48 ± 0.15 0.53 ± 0.22 1.09 ± 0.37 1.00 ± 0.17 0.86 ± 0.07 0.67 ± 0.20 0.51 ± 0.10 1.00 ± 0.02 0.93 ± 0.08 0.88 ± 0.09
Citrate 1.00 ± 0.15 1.04 ± 0.15 1.09 ± 0.07 1.33 ± 0.03 1.00 ± 0.31 0.75 ± 0.05 0.74 ± 0.12 0.76 ± 0.08 1.00 ± 0.18 0.78 ± 0.03 0.44 ± 0.07
Fumarate 1.00 ± 0.19 2.22 ± 0.70 0.85 ± 0.11 0.57 ± 0.07 1.00 ± 0.14 0.80 ± 0.06 0.69 ± 0.05 0.84 ± 0.08 1.00 ± 0.08 1.10 ± 0.07 1.01 ± 0.05
Glycerate 1.00 ± 0.04 0.97 ± 0.20 0.82 ± 0.07 0.98 ± 0.05 1.00 ± 0.13 0.58 ± 0.04 0.82 ± 0.16 0.82 ± 0.08 n.d. n.d. n.d.
Isocitrate 1.00 ± 0.03 0.56 ± 0.04 0.39 ± 0.05 3.15 ± 1.80 1.00 ± 0.13 1.13 ± 0.14 0.72 ± 0.17 0.41 ± 0.09 1.00 ± 0.12 0.60 ± 0.07 0.52 ± 0.07
Malate 1.00 ± 0.04 2.14 ± 0.17 2.53 ± 0.36 1.33 ± 0.26 1.00 ± 0.11 0.58 ± 0.11 1.00 ± 0.15 1.88 ± 0.18 1.00 ± 0.13 1.26 ± 0.09 1.33 ± 0.13
Oxalate n.d. n.d. n.d. n.d. 1.00 ± 0.22 1.13 ± 0.11 0.23 ± 0.05 0.37 ± 0.10 1.00 ± 0.28 0.29 ± 0.02 0.26 ± 0.01
Quinate 1.00 ± 0.37 1.99 ± 0.49 1.89 ± 0.50 0.77 ± 0.16 1.00 ± 0.26 0.58 ± 0.04 0.63 ± 0.11 0.63 ± 0.17 1.00 ± 0.04 0.70 ± 0.07 0.75 ± 0.10
Shikimate 1.00 ± 0.06 0.89 ± 0.11 0.88 ± 0.04 0.61 ± 0.06 1.00 ± 0.17 0.79 ± 0.06 0.40 ± 0.07 0.47 ± 0.06 1.00 ± 0.09 0.41 ± 0.02 0.29 ± 0.02
Succinate 1.00 ± 0.17 0.93 ± 0.25 1.44 ± 0.36 1.09 ± 0.17 1.00 ± 0.18 0.44 ± 0.04 0.46 ± 0.06 0.37 ± 0.02 1.00 ± 0.25 0.35 ± 0.03 0.28 ± 0.02
Threonate 1.00 ± 0.13 0.64 ± 0.16 0.57 ± 0.06 0.52 ± 0.11 1.00 ± 0.29 0.42 ± 0.06 n.d. n.d. n.d. n.d. n.d.
Fru 1.00 ± 0.19 2.12 ± 0.66 23.35 ± 12.51 1.82 ± 0.32 1.00 ± 0.51 0.38 ± 0.04 0.35 ± 0.04 3.28 ± 2.14 1.00 ± 0.17 5.84 ± 3.37 10.52 ± 6.15
Gal 1.00 ± 0.07 1.39 ± 0.24 1.01 ± 0.16 0.94 ± 0.10 1.00 ± 0.37 1.15 ± 0.21 0.71 ± 0.07 1.40 ± 0.34 n.d. 192.50 ± 26.22 282.17 ± 41.34
Glc 1.00 ± 0.26 2.30 ± 0.59 4.46 ± 0.58 1.34 ± 0.30 1.00 ± 0.35 1.02 ± 0.31 1.79 ± 0.40 2.72 ± 0.37 1.00 ± 0.18 1.18 ± 0.21 1.18 ± 0.14
Inositol 1.00 ± 0.15 1.29 ± 0.31 0.88 ± 0.05 0.51 ± 0.02 1.00 ± 0.24 0.47 ± 0.04 0.52 ± 0.05 0.40 ± 0.06 1.00 ± 0.06 0.58 ± 0.03 0.88 ± 0.05
Mannitol 1.00 ± 0.04 1.12 ± 0.14 1.81 ± 0.37 0.43 ± 0.04 1.00 ± 0.08 0.68 ± 0.02 0.52 ± 0.04 1.45 ± 0.13 1.00 ± 0.12 1.97 ± 0.18 3.07 ± 0.52
Man 1.00 ± 0.05 1.43 ± 0.19 1.44 ± 0.18 1.06 ± 0.16 1.00 ± 0.36 1.30 ± 0.20 0.85 ± 0.13 1.81 ± 0.29 1.00 ± 0.09 1.99 ± 0.13 2.90 ± 0.32
Suc 1.00 ± 0.06 1.20 ± 0.17 0.92 ± 0.08 1.18 ± 0.17 1.00 ± 0.35 1.58 ± 0.15 0.87 ± 0.23 1.89 ± 0.28 1.00 ± 0.14 1.16 ± 0.14 1.22 ± 0.10
Fru-6-P 1.00 ± 0.07 1.33 ± 0.10 1.50 ± 0.09 0.97 ± 0.04 1.00 ± 0.24 1.64 ± 0.19 1.38 ± 0.121 1.79 ± 0.16 1.00 ± 0.05 1.42 ± 0.07 1.65 ± 0.07
Glu-6-P 1.00 ± 0.04 1.43 ± 0.10 1.55 ± 0.09 0.86 ± 0.02 1.00 ± 0.25 1.33 ± 0.13 1.10 ± 0.07 1.83 ± 0.11 1.00 ± 0.14 2.31 ± 0.12 2.82 ± 0.20
3-PGA 1.00 ± 0.11 1.70 ± 0.12 2.04 ± 0.30 1.62 ± 0.42 1.00 ± 0.28 1.29 ± 0.15 1.30 ± 0.19 1.42 ± 0.31 n.d. n.d. n.d.
6-P-Gluconate 1.00 ± 0.22 1.63 ± 0.40 0.86 ± 0.23 1.72 ± 0.53 n.d. n.d. n.d. n.d. n.d. n.d. n.d.
Phosphate 1.00 ± 0.04 0.97 ± 0.03 0.92 ± 0.06 1.06 ± 0.02 1.00 ± 0.07 0.88 ± 0.07 0.80 ± 0.08 0.81 ± 0.06 1.00 ± 0.05 0.75 ± 0.06 0.57 ± 0.03

Data are normalized to the mean response calculated for the wild type of each measure batch. (To allow comparison between measure batches, individual wild-type values were normalized in the same way.) Values presented are the mean ± se of the mean of six independent determinants. Those that are significantly different from wild type are identified in bold type. n.d., Compounds that were not determined in a particular set of chromatograms; 3-PGA, 3-phosphoglyceric acid; GABA, 4-amino butyric acid.