Determination of tuber metabolite compositions in TS antisense plants. Polar metabolites were extracted from sink tuber parenchyma tissue of 8-week-old control plants (wt) and the TS antisense transgenic lines (five samples each). Metabolites were determined using GC/mass spectrometry. Actual concentrations of Met, Thr, Asp, Lys, Ile, and Glu were determined using external standards for calibration. Statistically significant changes (P < 0.05) are identified with an asterisk.