Figure 7.

In vitro bioassays of plants grown in the absence of spectinomycin. Five microliters of an overnight culture of P. syringae pv tabaci diluted to A₆₀₀ 0.1 to 0.3 was mixed with 100 μg of total protein extract from T₂ lines 11A and 13A (germinated in the absence of spectinomycin). After 2 h incubation, 50 μL of each mix was plated onto LB plates and incubated overnight at 27°C. A, A₆₀₀ was recorded the next morning using a spectrophotometer. B, Count of viable colony forming units (CFUs) were made using the Gell Dock (Bio-Rad, Hercules, CA).