In vitro antifungal bioassays in T1 transgenic lines. A, Inhibition of germinated conidia of A. flavus by exposure to leaf extracts from tobacco plants expressing the antifungal peptide MSI-99 for 1 h. Asterisk, Denotes a significant reduction (P < 0.001) in the number of A. flavus colonies compared to extracts of non-transformed control. B, Inhibition of germinated conidia of F. moniliforme by exposure to leaf extracts from tobacco plants expressing the antifungal peptide MSI-99 for 1 h. Asterisk, Denotes a significant reduction (P < 0.001) in the number of F. moniliforme colonies compared with extracts of non-transformed control. C, Inhibition of germinated conidia of V. dahliae by exposure to leaf extracts from tobacco plants expressing the antifungal peptide MSI-99 for 1 h. Asterisk, Denotes a significant reduction (P < 0.001) in the number of V. dahliae colonies compared with extracts of non-transformed control. Error bars indicate se of means. Mean separation was performed using the method of Tukey.