Figure 9.
In planta bioassays. Five- to 7-mm areas of T0 transformants and non-transformed tobacco cv Petit Havana leaves were scraped with fine-grain sandpaper. Ten microliters of 8 × 105, 8 × 104, 8 × 103, and 8 × 102 cells from an overnight culture of P. syringae pv tabaci were added to each prepared area. Photos were taken 5 d after inoculation. A, Transgenic leaf; B, wild-type leaf; C, transgenic; D, wild type were injected with 25 μL of 8 × 103 cells of Pst. Pictures were taken 5 d after inoculation. Transformed leaf show only slight discoloration, whereas the wild-type leaf shows necrosis.