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Cooperative induction of mRNA of the ER chaperones in Arabidopsis by tunicamycin. Total RNA was extracted from Arabidopsis seedlings after addition of tunicamycin (5 μg mL−1) to the culture medium. Incubation periods after treatment were 0 h (Control), 1 h (Tm 1 h), and 2.5 h (Tm 2.5 h). The RNA blot was probed with the cDNAs of BiP, PDI, calnexin, calreticulin-1, and calreticulin-2.
