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. 2026 Feb 9;17:1748950. doi: 10.3389/fphar.2026.1748950

FIGURE 7.

Bar chart A) shows the expression levels of CD44 with different treatments: Control, Dox (1μM), Dox + HMPSNE, and HMPSNE. Dox treatment shows a tendency of increase in CD44 expression, while co‐treatment with Dox+HMPSNE shows significant decrease in CD44 expression compared to Dox treatment alone. Flow cytometry histograms B) display CD44 fluorescence intensity for the same treatment groups and an unstained control. The table lists median fluorescence intensities and relative standard deviations (rSD) for each group.

Effects of HMPSNE (100 µM) alone or in combination with doxorubicin (1 µM) on CD44 expression in MDA-MB-231 cells. (A) qRT‒PCR was performed, and the results were normalized to those of B-actin as an internal control. The data are shown as the means ± SEMs of three independent experiments: *P < 0.05, **P < 0.01. (B) Representative curves for flow cytometry analysis of TNBC cells before and after treatment. The overlay shows CD44 expression upon treatment with doxorubicin and HMPSNE in comparison with the unstained control (orange), which was used to establish baseline autofluorescence and gating thresholds. The median fluorescence values with relative standard deviations are shown in the table under the figure. This experiment was repeated three times.