Table II.

Kinetic parameters of mutants in the proposed catalytic pair of Zm-p60.1 with MUG as substrate

Enzyme	Km	kcat(WT)/kcat(mut)
	mm
WT	0.14 ± 0.04	–
E186D	0.17 ± 0.03	8  × 103
E186Q	0.19 ± 0.03	1.5  × 103
E401D	0.16 ± 0.04	50a (1 × 104)b
E401Q	0.17 ± 0.04	4  × 104

k_cat(WT) was 140.5 ± 3.7 s⁻¹ · k_cat(mut) were estimated with accuracy comparable to k_cat(WT).

^a k_cat(E401D) related to dimer form. 

^b k_cat(E401D) related to mixture of dimer and monomer forms.