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Kinetic parameters of mutants in F193 and M258 of Zm-p60.1 with PNPG as substrate
| Enzyme | Km (mM) | kcat(mut)/kcat(WT) | [kcat/Km(mut)]/[kcat/Km(WT)] |
|---|---|---|---|
| WT | 0.64 ± 0.08 | – | – |
| F193I | 1.76 ± 0.06 | 0.03 | 0.01 |
| F193Y | 1.29 ± 0.01 | 0.62 | 0.31 |
| F193W | 1.61 ± 0.17 | 1.12 | 0.45 |
| M258I | 1.18 ± 0.36 | 0.76 | 0.41 |
| M258V | 0.59 ± 0.08 | 0.34 | 0.37 |
| M258F | 0.59 ± 0.08 | 0.30 | 0.33 |
kcat(WT) was 28.0 ± 0.8 s−1 · kcat(mut) were estimated with accuracy comparable to kcat(WT).
