Table IV.
Mouth opening calculated for Zm-p60.1 mutants in F193 and M258
| Enzyme | Solvent-Accessible Surface Area | Molecular Surface Area |
|---|---|---|
| Å2 | ||
| WT | 14 | 94 |
| F193I | 23 | 113 |
| F193Y | 10 | 78 |
| F193W | 11 | 88 |
| M258I | 16 | 94 |
| M258V | 40 | 135 |
| M258F | n.a.a | n.a.a |
Substitutions were introduced in the structure using INSIGHTII v95 (Biosym/MSI Accelrys). Solvent-accessible (Lee and Richards, 1971) and molecular (Connolly, 1983) surfaces of the mouth openings were calculated using CASTP v1.1 (Liang et al., 1998) with a solvent probe diameter of 1.4 Å.
a
Opposite trends in change of active site size were obtained for two distinct rotamers in M258F; thus, unambiguous values are not available (n.a.) for the mutant.