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. 2001 Nov;127(3):986–997.

Figure 2.

Figure 2

Effect of light on expression of Elips. A, RT-PCR analysis of 4-d-old Arabidopsis dark-grown wild-type seedlings that were kept in the dark or exposed to 1 h of white, blue, red, or far-red lights, or to heat shock in the dark at 37°C. One-fifth of the PCR products were resolved in 1.7% (w/v) agarose gel and blotted onto Hybond N+. The membrane was hybridized with DIG labeled Elip1 and Elip2 probes. B, RT-PCR LightCycler analysis reveals the differences between Elip1 and Elip2 transcript levels. Four-day-old Arabidopsis dark-grown wild-type seedlings were kept in the dark (D) or exposed to 1 h of white light (L) before total RNA extraction. One-tenth of the cDNA was amplified by the LightCycler. Top, Amplification curves of Elip1 and Elip2. The diagram documents the fluorescence intensity (approximate PCR product concentration) plotted against the number of PCR cycles. Bottom, Melting curve analysis of PCR products. The diagram documents the negative derivative fluorescence plotted against temperature, where the peak therefore highlights the melting of DNA.