Figure 3.
RT-PCR analysis of Elip2 expression in blue-light receptor mutants. Four-day-old Arabidopsis dark-grown wild-type (w.t.), cry1, cry2, cry1/cry2, nph1, and phyA/phyB seedlings strains were kept in the dark or exposed to 1 h of blue light before total RNA extraction. One-tenth of the RT-reaction was amplified by the LightCycler using Elip2 and Ubq10 primers. The normalized initial amount of Elip2 mRNA is shown.