Figure 5.

Monosaccharide composition of wild-type and mutant seeds and mucilage. The bars represent the average quantity of monosaccharide found by GC over multiple experiments. The data were standardized and converted to micrograms per 100 seed through comparison to an internal standard. The monosaccharide assignments were determined through coupled GC-MS. The occurrence of a monosaccharide in more than one bar is due to the sensitivity of GC analysis, where each anomer of each sugar is detected separately (Chaplin, 1986). Where anomers of a sugar were found in GC peaks representing a single monosaccharide, they were added to give a single bar. If an anomer of a sugar could not be resolved from that of a different monosaccharide, they were included as a separate, mixed bar. A, Comparison of the monosaccharide composition observed using GC of whole, ground seeds of wild-type Col (white bars), mum1-1 (striped bars), and mum2-1 (black bars). B, Comparison of the monosaccharide composition observed using GC for whole, ground seeds of wild-type Landsberg erecta (Ler; white bars), ttg1-1 (striped bars), and ap2-1 (black bars). C, Comparison of the monosaccharide composition observed using GC for extruded mucilage of wild-type Col (white bars), mum3-1 (striped bars), and mum5-1 (black bars) seeds. Analyses were done in triplicate for all genotypes except Col mucilage, which was done five times and Col whole, ground seeds, which was done four times. Error bars represent sd.