Table I.
Results of reciprocal backcrosses to wild-type plants and segregation analysis
| Mutant | F1 Seed Phenotypeab
|
F2 Seed Phenotype
|
F3 Seed
|
|||
|---|---|---|---|---|---|---|
| × WTc | WT × | × WT | WT × | WT:mutant | Chi squared | |
| mum1-1 | muc− | + | + | + | 188:50 | 2.0224, P > 0.1 |
| mum2-1 | muc− | + | + | + | 98:33 | 0.0025, P > 0.5 |
| mum3-1 | red.muc. | + | + | + | 57:20 | 0.0390, P > 0.5 |
| mum4-1 | muc− | + | + | + | 147:37 | 2.3480, P > 0.1 |
| mum5-1 | red.muc. | + | + | + | 142:41 | 0.6576, P > 0.1 |
a
F1 seed, Seed from cross; F2 seed, seed of F1 plants; F3 seed, seed of F2 plants.
b
Ruthenium red staining result; +, mucilage; muc−, no mucilage; red.muc., reduced mucilage.
c
All crosses written female parent × male parent; WT, wild type.
d
Null hypothesis of 3:1 WT:mutant; degrees of freedom = 1; cutoff at P = 0.05.