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. 2001 Nov;127(3):1020–1029.

Figure 3.

Figure 3

Intracellular localization of HA:sCAX1 and HA:lCAX1 in K667 mutant yeast. Yeast microsomal membranes were extracted and fractionated through a 15% to 50% (w/w) Suc gradient and 1-mL fractions were collected. Approximately 2 μg of protein from each of the seven fractions from 21% to 41% (w/w) Suc were separated by SDS-PAGE, blotted, then subjected to western-blot analyses using the anti-HA monoclonal antibody (HA:sCAX1 and HA:lCAX1) and an antibody against a yeast vacuolar membrane marker alkaline phosphatase (ALP).