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Time course of activase and Rubisco unfolding/aggregation during an increase in temperature. At time zero, purified activase (●) or Rubisco (○) were added to assays at 25°C. Over the next 20 min, the temperature of the assays (−) was increased to 65°C and then held constant at this temperature for an additional 20 min. Light scattering was measured over the entire time course. Light scattering by activase was measured in the presence of 0.75 mm ATPγS.
