Figure 4.

Enhanced aggregation of purified activase after thermal denaturation and trapping with rhodanese. Purified activase was incubated at the indicated temperatures in the presence of 0.75 mm ATPγS. After 10 min, chemically denatured rhodanese or guanidine-HCl alone was added to the reactions and the reactions were incubated for 5 min at 23°C. Aggregated protein was collected by centrifugation, separated by SDS-PAGE, and visualized by staining with Coomassie Brilliant Blue. The arrows labeled A and Rdn indicate the positions of activase (42 kD) and rhodanese (33.3 kD), respectively, on the gel.