Figure 5.

Effect of temperature and ATPγS on rhodanese trapping of heat-treated activase and Rubisco. Purified activase (black and shaded bars) or purified Rubisco (white bars) were incubated at the indicated temperatures in the absence (black and white bars) and presence (shaded bars) of 0.75 mm ATPγS. After 10 min, chemically denatured rhodanese was added to the reactions and the reactions were incubated for 5 min at 23°C. Aggregated protein was collected by centrifugation, separated by SDS-PAGE and visualized by staining with Coomassie Brilliant Blue. The amount of activase and Rubisco that aggregated was determined by densitometry using known amounts of the purified proteins as standards.