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. 2001 Nov;127(3):1053–1064.

Figure 6.

Figure 6

Enhanced aggregation of thermally denatured activase from heat-treated tobacco protoplasts by trapping with rhodanese. A, Intact protoplasts were incubated for 10 min at the indicated temperatures and then lysed in detergent in the presence (+) and absence (−) of rhodanese. B, Intact protoplasts were incubated for 2 and 10 min at 48°C and then lysed with detergent in the presence of rhodanese. After 5 min at 23°C, aggregated protein was collected by centrifugation, separated by SDS-PAGE, and the activase polypeptides were visualized by immunoblotting. The arrows on the blot indicate the position of the 42-kD activase polypeptide.