Figure 6.
Identification of grapevine peroxidases with high capacity for the cross-linking of GvP1 extensin. A, In vitro assay of extensin cross-linking by grapevine peroxidases. The different peroxidases and their quantities (pmole) assayed are indicated to the right of the figure. The elution volumes for the different protein species are indicated at the top. B, In situ assay for GvP1 extensin deposition by grapevine peroxidases. The different peroxidases assayed are indicated to the right of the figure. In all cases, 0.7 pmol of peroxidase mg−1 (fresh weight) cells were utilized, and KCl extracts from 35 mg (fresh weight) cells was analyzed. C, The relation of extensin deposition with the abundance of Px3 in cell walls.