Table IV.
Primer sequences used in this study
| Primer Name | Sequence (5′ → 3′) | Size (Col-0) | Size (Ler) |
|---|---|---|---|
| bp | |||
| recMS1f | GTGTGTCTATTAATATATCAAGAC | 125 | ∼120 |
| recMS1r | GTTGTTTTATCTATGTTTATCAAG | ||
| recMS2f | CCATTGGAGATGGTCTTACAC | 107 | ∼90 |
| recMS2r | CTTTGTCACCAAGTCTTGTATAG | ||
| recMS5f | GAATCAGATAATTAAGTTAGATAG | 106 | No product |
| recMS5r | GGTTCTTCATAAAAACAAAGCAC | ||
| rec6f | GTGTAGTTTCATTTGTGCTATC | 75 | 147 |
| rec6r | CTTTAAAACTCTAAGCCTAGAC | ||
| recMS7f | CATAAGATGGAAGCGTTAGAC | 132 | ∼150 |
| recMS7r | GATGGTAAACTACTTTGTGAAC | ||
| recMS9f | GGTTCCAAGTTACAAACTTTAAC | 105 | ∼90 |
| recMS9r | CGACCTAACGGCAAGTGAAAG | ||
| EN-MSfor | CATGAGAGCGGCATTCACCAG | 100 | 88 |
| EN-MSrev | GGACAATTATACACATACAACGC | ||
| 16Cm2 | GGGCTTATGTGCTTGAAC | 430 | 430 |
| Southex | GAAATGTCGAGGGGCATATG | SNPs, 1-bp indels | SNPs, 1-bp indels |
| EDIT1-2a | GTACCACAGTAATATCAATTTCC | 207 | 208 |
| 11t1 | GTTCATGAGATATGAGTAAGTGG | SNPs, 1-bp indel | SNPs, 1-bp indel |
| C/L-ESfEE | ATATGAATTCAACTATGTGCGTGTATTCGAC | – | – |
| CESrEE | ATATCTCGAGCACATTCGATGAAACCTGAGG | – | – |
| L-ENfEE | ATATGGATCCTATGTGCGTGTATTAGACAC | – | – |
| L-ENrEE | ATATCTCGAGTACAACAGCGGAAATCTGAGG | – | – |
| IPMS0 | CACCGTCACGTTGAAACCTTC | – | – |
| E116r | TTTTTAATTCAGGCAGCGACTCT | – | – |
| 1MAM1a | ACTCCATAGTGATGGCTTCATCGCTTCTG | – | – |
| 2MAM1b | AGAGATACAACGTGTTTACACATTC | – | – |
| 1MAMLa | TATAGTAATGGCTTCGTTACTTCTC | – | – |
| 2MAMLb | TCTTCCCAAACTTATACAACAGCG | – | – |
Size is indicated for PCR products used as genetic markers.