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Biological induction of hydrogen peroxide accumulation in ndr1-1 versus Columbia. Columbia or ndr1-1 plants were inoculated with P. syringae pv tomato DC3000 carrying a plasmid-borne copy of avrRpt2, avrB, or only the empty pVSP61 vector. DCFH-DA was inoculated 15 to 30 min prior to excising of the leaves at the indicated time points for brief exposure to 365 nm of UV light followed immediately by photography.
